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FLAG-HA-FbxO5-pcDNA3.1-
(Plasmid #52509)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52509 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1-
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5489
  • Total vector size (bp) 6840
  • Modifications to backbone
    added FLAG-HA tag in front of MCS, tag coding sequence has: Kozak – FLAG - 5x Glycine – HA – 5x Glycine – MCS ...
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FbxO5
  • Alt name
    EMI1
  • Alt name
    Fbxo31
  • Alt name
    Fbx5
  • Species
    H. sapiens (human); FbxO5
  • Insert Size (bp)
    1351
  • GenBank ID
    NM_012177.3
  • Entrez Gene
    FBXO5 (a.k.a. EMI1, FBX5, Fbxo31)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG-HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba I (not destroyed)
  • 3′ cloning site Not I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH-polyA reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    insert cDNA was amplified from DF/HCC PlasmID # HsCD00040868 Accession: BC018905
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-HA-FbxO5-pcDNA3.1- was a gift from Adam Antebi (Addgene plasmid # 52509 ; http://n2t.net/addgene:52509 ; RRID:Addgene_52509)
  • For your References section:

    DRE-1/FBXO11-Dependent Degradation of BLMP-1/BLIMP-1 Governs C. elegans Developmental Timing and Maturation. Horn M, Geisen C, Cermak L, Becker B, Nakamura S, Klein C, Pagano M, Antebi A. Dev Cell. 2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 10.1016/j.devcel.2014.01.028. 10.1016/j.devcel.2014.01.028 PubMed 24613396