Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #52509)


Item Catalog # Description Quantity Price (USD)
Plasmid 52509 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5489
  • Total vector size (bp) 6840
  • Modifications to backbone
    added FLAG-HA tag in front of MCS, tag coding sequence has: Kozak – FLAG - 5x Glycine – HA – 5x Glycine – MCS ...
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Alt name
  • Alt name
  • Species
    H. sapiens (human); FbxO5
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    FBXO5 (a.k.a. EMI1, FBX5, Fbxo31)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG-HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba I (not destroyed)
  • 3′ cloning site Not I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH-polyA reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    insert cDNA was amplified from DF/HCC PlasmID # HsCD00040868 Accession: BC018905
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-HA-FbxO5-pcDNA3.1- was a gift from Adam Antebi (Addgene plasmid # 52509 ; ; RRID:Addgene_52509)
  • For your References section:

    DRE-1/FBXO11-Dependent Degradation of BLMP-1/BLIMP-1 Governs C. elegans Developmental Timing and Maturation. Horn M, Geisen C, Cermak L, Becker B, Nakamura S, Klein C, Pagano M, Antebi A. Dev Cell. 2014 Mar 4. pii: S1534-5807(14)00069-0. doi: 10.1016/j.devcel.2014.01.028. 10.1016/j.devcel.2014.01.028 PubMed 24613396