PurposeTransient over-expression of human Axin1
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||109370||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4324
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneAXIN1 (a.k.a. AXIN, PPP1R49)
- Promoter CMV
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Flag-Axin1 was a gift from Mariann Bienz (Addgene plasmid # 109370 ; http://n2t.net/addgene:109370 ; RRID:Addgene_109370)
For your References section:Dishevelled interacts with the DIX domain polymerization interface of Axin to interfere with its function in down-regulating beta-catenin. Fiedler M, Mendoza-Topaz C, Rutherford TJ, Mieszczanek J, Bienz M. Proc Natl Acad Sci U S A. 2011 Feb 1;108(5):1937-42. doi: 10.1073/pnas.1017063108. Epub 2011 Jan 18. 10.1073/pnas.1017063108 PubMed 21245303