pcDNA3 V5 hHR23B
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13054||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepcDNA3.1D V5 His TOPO
- Backbone size w/o insert (bp) 5500
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1200
Entrez GeneRAD23B (a.k.a. HHR23B, HR23B, P58)
/ Fusion Proteins
- V5 (C terminal on backbone)
- His (C terminal on backbone)
- Cloning method TOPO Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer BGH rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
You can cut out the hHR23B insert with HindIII and XhoI.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3 V5 hHR23B was a gift from Steven Grossman (Addgene plasmid # 13054 ; http://n2t.net/addgene:13054 ; RRID:Addgene_13054)
For your References section:A post-ubiquitination role for MDM2 and hHR23A in the p53 degradation pathway. Brignone C, Bradley KE, Kisselev AF, Grossman SR. Oncogene. 2004 May 20. 23(23):4121-9. 10.1038/sj.onc.1207540 PubMed 15064742