p3998 pGEX HHR23A
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10864||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4970
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneRAD23A (a.k.a. HHR23A, HR23A)
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer pGEX5' (Common Sequencing Primers)
See author's map.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p3998 pGEX HHR23A was a gift from Peter Howley (Addgene plasmid # 10864 ; http://n2t.net/addgene:10864 ; RRID:Addgene_10864)
For your References section:Identification of HHR23A as a substrate for E6-associated protein-mediated ubiquitination. Kumar S, Talis AL, Howley PM. J Biol Chem. 1999 Jun 25. 274(26):18785-92. 10.1074/jbc.274.26.18785 PubMed 10373495
Map uploaded by the depositor.