p3869 HA-E6-AP C833A
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8649||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4874
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)2500
MutationC833A; active site Cys mutated to encode Ala
Entrez GeneUBE3A (a.k.a. ANCR, AS, E6-AP, EPVE6AP, HPVE6A)
/ Fusion Protein
- HA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
See author's map. In addition to C833A, there is also an L837V amino acid change. E6-AP is missing the first 22 amino acids of isoform 1 (protein starts with MEACT NEFCA).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p3869 HA-E6-AP C833A was a gift from Peter Howley (Addgene plasmid # 8649 ; http://n2t.net/addgene:8649 ; RRID:Addgene_8649)
For your References section:The role of E6AP in the regulation of p53 protein levels in human papillomavirus (HPV)-positive and HPV-negative cells. Talis AL, Huibregtse JM, Howley PM. J Biol Chem 1998 Mar 13;273(11):6439-45. 10.1074/jbc.273.11.6439 PubMed 9497376
Map uploaded by the depositor.