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Addgene

p4028 GST-E6AP
(Plasmid #8655)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8655 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGEX-2T
  • Backbone size w/o insert (bp) 4948
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E6-AP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2500
  • Entrez Gene
    UBE3A (a.k.a. ANCR, AS, E6-AP, EPVE6AP, HPVE6A, PIX1)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer pGEX-5'
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map. E6-AP is missing the first 22 amino acids of isoform 1 (protein starts with MEACT NEFCA).

There is also an N->K mutation 20 amino acids downstream from the beginning of the insert. This mutation has no known functional ramifications.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p4028 GST-E6AP was a gift from Peter Howley (Addgene plasmid # 8655 ; http://n2t.net/addgene:8655 ; RRID:Addgene_8655)
  • For your References section:

    Regulation of the Src family tyrosine kinase Blk through E6AP-mediated ubiquitination. Oda H, Kumar S, Howley PM. Proc Natl Acad Sci U S A 1999 Aug 17;96(17):9557-62. 10.1073/pnas.96.17.9557 PubMed 10449731