|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8655||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4948
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)2500
Entrez GeneUBE3A (a.k.a. ANCR, AS, E6-AP, EPVE6AP, HPVE6A)
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (destroyed during cloning)
- 5′ sequencing primer pGEX-5' (Common Sequencing Primers)
See author's map. E6-AP is missing the first 22 amino acids of isoform 1 (protein starts with MEACT NEFCA).
There is also an N->K mutation 20 amino acids downstream from the beginning of the insert. This mutation has no known functional ramifications.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p4028 GST-E6AP was a gift from Peter Howley (Addgene plasmid # 8655 ; http://n2t.net/addgene:8655 ; RRID:Addgene_8655)
For your References section:Regulation of the Src family tyrosine kinase Blk through E6AP-mediated ubiquitination. Oda H, Kumar S, Howley PM. Proc Natl Acad Sci U S A 1999 Aug 17;96(17):9557-62. 10.1073/pnas.96.17.9557 PubMed 10449731