|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13465||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4948
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)2500
MutationC833A; active site Cys mutated to encode Ala
Entrez GeneUBE3A (a.k.a. ANCR, AS, E6-AP, EPVE6AP, HPVE6A)
/ Fusion Protein
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (destroyed during cloning)
- 5′ sequencing primer pGEX-5' (Common Sequencing Primers)
In addition to C833A, there is also an L837V amino acid change. E6-AP is missing the first 22 amino acids of isoform 1 (protein starts with MEACT NEFCA).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:GST-E6AP C833A was a gift from Michael Greenberg (Addgene plasmid # 13465)