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pREP-NFAT4
(Plasmid #11790)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11790 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 11790-D50 50 μg of DNA in Tris buffer $495
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DNA 11790-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pREP4
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 10200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NFAT4
  • Alt name
    NFAT4a isoform
  • Species
    H. sapiens (human)
  • Entrez Gene
    NFATC3 (a.k.a. NF-AT4c, NFAT4, NFATX, n339260)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Hoey et al, 1995, PMID 7749981.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this plasmid contains the NFAT4a isoform as described in the following publication: https://www.ncbi.nlm.nih.gov/pubmed/7749981 This isoform contains 708 amino acids.

Please note: Addgene NGS is unable to fully resolve the length of the poly-A tail in the 3'UTR

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pREP-NFAT4 was a gift from Anjana Rao (Addgene plasmid # 11790 ; http://n2t.net/addgene:11790 ; RRID:Addgene_11790)
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