166,818 results

1. SBFP2-anti-AlfaTag nanobody

   Plasmid

   #171826

   Purpose

   Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

   Depositor

   Ikuo Wada

   Article

   Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

   Insert

   SBFP2-anti-AlfaTag nanobody
   

   Use

   Tags

   6xHis and SEP tag (6xD)

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3.1 (-) + codon optimized Brn3a

   Plasmid

   #86829

   Purpose

   mammalian expression of the transcription factor Brn3a

   Depositor

   Carl Novina

   Article

   Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

   Insert

   Brn3a (POU4F1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-P4M-SidM

   Plasmid

   #51469

   Purpose

   Biosensor for PtdIns4P

   Depositor

   Tamas Balla

   Article

   Hammond et al J Cell Biol. 2014 Apr 7.

   Insert

   P4M-SidM(546-647)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS hTSP-5

   Plasmid

   #13002

   Depositor

   Jack Lawler

   Article

   Newton et al Genomics. 1994 Dec . 24(3):435-9.

   Insert

   COMP (COMP Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 28, 2006

   Availability

   Academic Institutions and Nonprofits only

5. lentiGuideFE-Puro

   Plasmid

   #170069

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element (with F+E modifications) with customizable gRNA from U6 promoter and puromycin resistance from EFS-NS promoter

   Depositor

   Neville Sanjana

   Article

   Morris et al Science. 2023 May 19;380(6646):eadh7699.

   Insert

   Cas9 guide RNA scaffold with the F+E scaffold modification
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-FLEX-EGFP-KASH

   Plasmid

   #154373

   Purpose

   Vector for Cre-dependent expression of EGFP tagged KASH protein domain

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   EGFP-KASH
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCW57.1_AOX-FLAG

   Plasmid

   #177984

   Purpose

   All-in-one dox-inducible expression of Alternative Oxidase

   Depositor

   Jessica Spinelli, David Sabatini

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   AOX
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Codon optimized cDNA for expression in human cells

   Promoter

   TRE

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET30a-Tn5

   Plasmid

   #127917

   Purpose

   Prokaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.

   Depositor

   Silin Zhong

   Article

   Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

   Insert

   Tn5
   

   Use

   Tags

   Mxe intein - Chitin-binding domain

   Expression

   Bacterial

   Mutation

   E54K, L372P

   Promoter

   T7

   Available Since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET30-2-GPD1

   Plasmid

   #83911

   Purpose

   stable overexpression

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   glycerol-3-phosphate dehydrogenase 1 (GPD1 Human)
   

   Use

   Tags

   6x His tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. CROPseq-puro-v2

    Plasmid

    #127458

    Purpose

    Entry plasmid for guide cloning

    Depositor

    Paul Blainey

    Article

    Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAW63.YY1.FKBP.knock-in.BFP

    Plasmid

    #104371

    Purpose

    For knocking in FKBP F36V P2A BFP

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Insert

    FKBP12(F36V) -P2A-BFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-RAPH1ΔTBS

    Plasmid

    #194859

    Purpose

    Express eGFP-RAPH1 lacking the TBS domain (RAPH1 aa 2-92 deleted) in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

    Insert

    RAPH1 (RAPH1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    RAPH1 with amino acids 2-92 deleted

    Promoter

    CMV

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (AAV Retrograde)

    Viral Prep

    #154867-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (#154867). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Ulrik Gether

    Article

    Runegaard et al eNeuro. 2018 Jun 18;5(3). pii: eN-NWR-03

    Promoter

    Human Synapsin-1

    Tags

    mCherry (Flp-dependent)

    Available Since

    Oct. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3-mRFP

    Plasmid

    #13032

    Purpose

    Mammalian expression vector for cloning your gene fused to mRFP

    Depositor

    Doug Golenbock

    Has Service

    Cloning Grade DNA

    Insert

    Monomer Red Fluorescent Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLAG-mBAF45a IRES GFP

    Plasmid

    #17880

    Depositor

    Jerry Crabtree

    Article

    Lessard et al Neuron. 2007 Jul 19. 55(2):201-15.

    Insert

    BAF45a (Phf10 Mouse)
    

    Use

    Tags

    FLAG and IRES EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-CMV-FlaviA-mNeptune-puro

    Plasmid

    #140091

    Purpose

    Fluorescence activatable reporter of flavivirus NS2B-NS3 protease activity

    Depositor

    Jorge Luis Arias-Arias

    Article

    Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

    Insert

    FlaviA-mNeptune
    

    Use

    Lentiviral; High expression

    Tags

    mNeptune

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21a(+)-Bst LF-6xHis

    Plasmid

    #159148

    Purpose

    expresses His-tagged large fragment of Bst polymerase

    Depositor

    Andrea Pauli

    Article

    Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d

    Insert

    large fragment of Geobacillus stearothermophilus DNA polymerase I
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pIRES-HA-14-3-3 ϵ/myc-14-3-3ζ

    Plasmid

    #48795

    Purpose

    expresses human HA-14-3-3 ϵ (epsilon) and myc-14-3-3ζ (zeta) in mammalian cells

    Depositor

    Huda Zoghbi

    Article

    Chen et al Cell. 2003 May 16;113(4):457-68.

    Inserts

    YWHAE (YWHAE Human)
    YWHAZ (YWHAZ Human)
    

    Use

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and CMV (IRES)

    Available Since

    May 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. GPR141-Tango

    Plasmid

    #66318

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR141 (GPR141 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pSD5

    Plasmid

    #197289

    Purpose

    Contains a seamless deletion cassette for the replacement of ORF2230 in D. radiodurans

    Depositor

    Bogumil Karas

    Article

    Brumwell et al Biodes Res. 2023 Mar 15;5:0009. doi: 10.

    Insert

    NptII
    

    Use

    Synthetic Biology; Bacterial and yeast cloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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