|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16076||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5190
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameHSP33 cys
Insert Size (bp)900
/ Fusion Proteins
- EYFP (N terminal on insert)
- ECFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byDr. Ursula Jakob, Univ of MI
Terms and Licenses
- Not Available to Industry
Article Citing this Plasmid
The HSP-FRET probe was generated by inserting YFP into the pECFP-N1 plasmid between the NheI and BglII sites and then ligating the redox-sensitive regulatory domain from the Escherichia coli HSP-33 between YFP and CFP via the EcoRI and BamHI sites. HSP-33: 210 amino acids; BA000007 region 4241962-4242826. Made in Hammerling lab; used in Waypa et al., Increases in mitochondrial reactive oxygen species trigger hypoxia-induced calcium responses in pulmonary artery smooth muscle cells, Circ Res. 2006 Oct 27; 99(9):970-8 PMID: 17008601.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFRET-HSP33 cys was a gift from Ulrich Hammerling (Addgene plasmid # 16076 ; http://n2t.net/addgene:16076 ; RRID:Addgene_16076)
Map uploaded by the depositor.