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pFRET-HSP33 cys
(Plasmid #16076)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 16076 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5190
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    HSP33 cys
  • Species
    E. coli
  • Insert Size (bp)
  • Tags / Fusion Proteins
    • EYFP (N terminal on insert)
    • ECFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The HSP-FRET probe was generated by inserting YFP into the pECFP-N1 plasmid between the NheI and BglII sites and then ligating the redox-sensitive regulatory domain from the Escherichia coli HSP-33 between YFP and CFP via the EcoRI and BamHI sites. HSP-33: 210 amino acids; BA000007 region 4241962-4242826. Made in Hammerling lab; used in Waypa et al., Increases in mitochondrial reactive oxygen species trigger hypoxia-induced calcium responses in pulmonary artery smooth muscle cells, Circ Res. 2006 Oct 27; 99(9):970-8 PMID: 17008601.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFRET-HSP33 cys was a gift from Ulrich Hammerling (Addgene plasmid # 16076 ; ; RRID:Addgene_16076)