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pCMX/cyclinB1-GFP (27)
(Plasmid #26061)


Item Catalog # Description Quantity Price (USD)
Plasmid 26061 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Cyclin B1
  • Species
    H. sapiens (human)
  • Entrez Gene
    CCNB1 (a.k.a. CCNB)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer GFP-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The stop codon of cyclin B1 in the pCMX vector was mutated to a Mfe1 site to link cyclin B1 to MmGFP. Cyclin B1 was fused to the second amino acid residue of MmGFP by a 5 amino acid linker (AGAQF). The construct is cloned as a HindIII-BamHI fragment into pCMX.

The Cyclin insert contains a V5A polymorphism which has no functional consequence. More information on MmGFP can be found in the following reference: Zernicka-Goetz, M., Pines, J., M., Ryan, K., Siemering, K.R., Haseloff, J., and Gurdon, J.B. (1996) ‘An indelible lineage marker for Xenopus using a mutated green fluorescent protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMX/cyclinB1-GFP (27) was a gift from Jonathon Pines (Addgene plasmid # 26061 ; ; RRID:Addgene_26061)
  • For your References section:

    Translocation of cyclin B1 to the nucleus at prophase requires a phosphorylation-dependent nuclear import signal. Hagting A, Jackman M, Simpson K, Pines J. Curr Biol. 1999 Jul 1. 9(13):680-9. 10.1016/S0960-9822(99)80308-X PubMed 10395539