|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26060||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4500
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameCyclin B1
SpeciesH. sapiens (human)
Entrez GeneCCNB1 (a.k.a. CCNB)
/ Fusion Protein
- Myc (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
There is a V5A mutation in cycB1. The mutation does not affect localization of the protein or it's degradation.
For a wild type source of CyclinB1, please see plasmid 26062.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMX/cyclinB1 (17) was a gift from Jonathon Pines (Addgene plasmid # 26060 ; http://n2t.net/addgene:26060 ; RRID:Addgene_26060)
For your References section:Translocation of cyclin B1 to the nucleus at prophase requires a phosphorylation-dependent nuclear import signal. Hagting A, Jackman M, Simpson K, Pines J. Curr Biol. 1999 Jul 1. 9(13):680-9. 10.1016/S0960-9822(99)80308-X PubMed 10395539