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pCMX/cyclinB1 (17)
(Plasmid #26060)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26060 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cyclin B1
  • Species
    H. sapiens (human)
  • Entrez Gene
    CCNB1 (a.k.a. CCNB)
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

There is a V5A mutation in cycB1. The mutation does not affect localization of the protein or it's degradation.

For a wild type source of CyclinB1, please see plasmid 26062.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMX/cyclinB1 (17) was a gift from Jonathon Pines (Addgene plasmid # 26060 ; http://n2t.net/addgene:26060 ; RRID:Addgene_26060)
  • For your References section:

    Translocation of cyclin B1 to the nucleus at prophase requires a phosphorylation-dependent nuclear import signal. Hagting A, Jackman M, Simpson K, Pines J. Curr Biol. 1999 Jul 1. 9(13):680-9. 10.1016/S0960-9822(99)80308-X PubMed 10395539