Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(R42A) Cyclin B1-GFP (62)
(Plasmid #61849)


Item Catalog # Description Quantity Price (USD)
Plasmid 61849 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    K.Umesono, Salk Institute
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    cyclin B1
  • Species
    H. sapiens (human)
  • Mutation
    V5A R42A
  • Entrez Gene
    CCNB1 (a.k.a. CCNB)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Myc (N terminal on insert)
    • GFP (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is an addition conservative mutation at position Val5 (Ala in this construct).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    (R42A) Cyclin B1-GFP (62) was a gift from Jonathon Pines (Addgene plasmid # 61849 ; ; RRID:Addgene_61849)
  • For your References section:

    Temporal and spatial control of cyclin B1 destruction in metaphase. Clute P, Pines J. Nat Cell Biol. 1999 Jun;1(2):82-7. 10.1038/10049 PubMed 10559878