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GFP-(SUMO)1

Plasmid

#127089

Purpose

Bacterial expression plasmid for eGFP-(SUMO)1 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

EGFP human SUMO3 (SUMO3 Human)

Tags

EGFP

Expression

Bacterial

Promoter

unknown

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pLVET-IRES-GFP

Plasmid

#107139

Purpose

A bicistronic lentiviral mammalian expression vector with a multiple cloning site and GFP

Depositor

Aki Manninen

Article

Zhang et al Oncogene. 2017 Oct 12;36(41):5681-5694.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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BTV

Plasmid

#131589

Purpose

Plasmid for the targeting of BAC clone

Depositor

Joon Tae Park

Insert

Firefly Luciferase

Expression

Mammalian

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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mEGFP-biRhoBAST

Plasmid

#196348

Purpose

Expression of mEGFP mRNA with biRhoBAST in 3'UTR

Depositor

Murat Sunbul

Article

Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

Insert

mEGFP-biRhoBAST

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCX459

Plasmid

#229214

Purpose

CRISPR array plasmid for MAP1B (Spacers 1-24)

Depositor

Jennifer Doudna

Article

Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

Insert

CRISPR array plasmid for MAP1B (Spacers 1-24)

Expression

Mammalian

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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PRS303-240xtetO

Plasmid

#44754

Depositor

Narendra Maheshri

Article

Finney-Manchester et al Nucleic Acids Res. 2013 Mar 7.

Insert

240x tetO array and CHRI homology

Expression

Yeast

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pFK210B-AtMIR390a-B/c

Plasmid

#51777

Purpose

Plant expression vector (35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor

Depositor

James Carrington

Article

Carbonell et al Plant Physiol. 2014 Mar 19.

Insert

AtMIR390a-B/c

Use

Plant expression

Mutation

A. thaliana MIR390a precursor sequence including …

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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pHTN-HaloTag_WDR91:1-747

Plasmid

#210996

Purpose

Mammalian expression of full-length human WDR91. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR91:M1-A747 (WDR91 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pRSETb MGlucoMeter2.6-1m

Plasmid

#247471

Purpose

Matryoshka-based Glucose Sensor

Depositor

Wolf Frommer

Article

Ishikawa et al bioRxiv 2025.09.27.678933; doi: 10.1101/

Insert

Glucose Binding Protein (GBP) with Matryoshka dual fluorophore cassette

Expression

Bacterial

Available Since

Feb. 13, 2026

Availability

Academic Institutions and Nonprofits only

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M13cp-dg2 hp

Plasmid

#218094

Purpose

Helper plasmid that allows phagemid production upon complementation with a phagemid expressing pII

Depositor

Kevin Esvelt

Article

Gretton et al bioRxiv 2024.03.20.585782

Type

Empty backbone

Expression

Bacterial

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pSAM_Bt

Plasmid

#112497

Purpose

Transposon mutagenesis vector for Bacteroides thetaiotaomicron. Compatible with Insertion Sequencing (INSeq).

Depositor

Andrew Goodman, Jeffrey Gordon

Article

Goodman et al Cell Host Microbe. 2009 Sep 17;6(3):279-

Inserts

INSeq-adapted mariner transposon
Himar1C9

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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SlugABEmax

Plasmid

#163798

Purpose

Expresses ABEmax and nSlugCas9

Depositor

Yongming Wang

Article

Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Mutation

SlugCas9 (D10A)

Available Since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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CREB3L3

Plasmid

#99509

Purpose

to express CREB3L3 in mammalian system

Depositor

Dong-Yan Jin

Article

Chin et al Nucleic Acids Res. 2005 Mar 30;33(6):185

Insert

CREB3L3 (Creb3l3 Mouse)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDC180

Plasmid

#218673

Purpose

Expresses rat ⍺SNAP in bacteria

Depositor

Axel Brunger

Article

Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

Insert

rat ⍺SNAP (Napa Rat)

Tags

6 his

Expression

Bacterial

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-Intron-CterFlag-MePCE

Plasmid

#113549

Purpose

Mammalian expression plasmid that can be used with the Flp-In T-REx system.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

Flag

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pIG-747pUC19-tNGFR-P2A-CD7

Plasmid

#186086

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CD7 (CD7 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-endo-EKAR4

Plasmid

#205513

Purpose

Genetically encoded FRET-based biosensor for monitoring ERK kinase activity. Targeted to endosomes.

Depositor

Jin Zhang

Article

Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

Insert

endo-EKAR4

Tags

2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and 6xHI…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pRDA_174

Plasmid

#136476

Purpose

Lentiviral expression of EnAsCas12a

Depositor

John Doench

Article

DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

Insert

EnAsCas12a

Use

CRISPR and Lentiviral

Promoter

EF1a

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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eNme2C-CBE6a

Plasmid

#215829

Purpose

Express CBE6a (with eNme2-C variant) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6a-eNme2C-2xUGI

Expression

Mammalian

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pPMVAu5 (R)

Plasmid

#98296

Purpose

Enhancing the upper-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.

Depositor

Claudia Vickers

Article

Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

Insert

PSSA1-ERG10-TNAT1-PRPL4A-ERG13-TEFM1-PRPL15A-tHMG1-TEBS1

Expression

Yeast

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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