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GFP-Mfn1
(Plasmid #141154)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 141154 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAcGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4707
  • Total vector size (bp) 6933
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mitofusin 1
  • Alt name
    MFN1
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_033540
  • Entrez Gene
    MFN1 (a.k.a. hfzo1, hfzo2)
  • Promoter CMV
  • Tag / Fusion Protein
    • AcGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer pFastBac Reverse
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-Mfn1 was a gift from Gia Voeltz (Addgene plasmid # 141154 ; http://n2t.net/addgene:141154 ; RRID:Addgene_141154)
  • For your References section:

    Fission and fusion machineries converge at ER contact sites to regulate mitochondrial morphology. Abrisch RG, Gumbin SC, Wisniewski BT, Lackner LL, Voeltz GK. J Cell Biol. 2020 Apr 6;219(4). pii: 133843. doi: 10.1083/jcb.201911122. 10.1083/jcb.201911122 PubMed 32328629