232,579 results

1. Flag-SIRT1 H363Y

   Plasmid

   #1792

   Depositor

   Michael Greenberg

   Article

   Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

   Insert

   SIRT1 (SIRT1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   H363Y, deacetylase domain mutation

   Available Since

   May 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. Flag-SIRT1 H363Y

   Plasmid

   #1792

   Depositor

   Michael Greenberg

   Article

   Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

   Insert

   SIRT1 (SIRT1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   H363Y, deacetylase domain mutation

   Available Since

   May 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAGM51323

   Plasmid

   #153224

   Purpose

   Level 1 position 2 containing the Arabidopsis Rps5a promoter, Cas9 (Zcasi), and the nos terminator

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Insert

   Arabidopsis Rps5a promoter, Cas9 (Zcasi), nos terminator
   

   Use

   Synthetic Biology

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Recall-miniCMV-sfGFP

   Plasmid

   #137995

   Purpose

   Backbone for generation of ClonMapper Recall Plasmid

   Depositor

   Amy Brock

   Article

   Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPRyl_XPR2

   Plasmid

   #84609

   Purpose

   Introduces DSB at XPR2 locus in Y. lipolytica

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2016 Dec 19.

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Promoter

   UAS1B8-TEF(136)

   Available Since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPRyl_XPR2

   Plasmid

   #84609

   Purpose

   Introduces DSB at XPR2 locus in Y. lipolytica

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2016 Dec 19.

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Promoter

   UAS1B8-TEF(136)

   Available Since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LTR5HS S. pyogenes scaffold CARGO array

   Plasmid

   #191316

   Purpose

   Contains an array of guide RNAs targeting LTR5Hs elements

   Depositor

   Joanna Wysocka

   Article

   Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

   Insert

   Array of guide RNAs
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSLQ9926: pHR-PGK-SV40_NLS-dCasMINI-V4-VPR-c- Myc_NLS-mCherry-WPRE

   Plasmid

   #176269

   Purpose

   Expression of dCasMINI-V4-VPR-mCherry in mammalian cells

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   dCasMINI-V4-VPR-mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGWB455

   Plasmid

   #74837

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGWB455

    Plasmid

    #74837

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDTA-TK

    Plasmid

    #22677

    Depositor

    Robert Benezra

    Article

    Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

    Insert

    DTA and TK
    

    Use

    Mouse Targeting

    Mutation

    pDTA-TK dual selection plasmid for targeting vect…

    Available Since

    Dec. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDTA-TK

    Plasmid

    #22677

    Depositor

    Robert Benezra

    Article

    Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

    Insert

    DTA and TK
    

    Use

    Mouse Targeting

    Mutation

    pDTA-TK dual selection plasmid for targeting vect…

    Available Since

    Dec. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPHR-mCherry-hNCL

    Plasmid

    #181886

    Purpose

    Soluble light-dependent effector protein (nucleolin) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-hNCL (NCL Mustard Weed, Human)
    

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a_TIA1

    Plasmid

    #106095

    Purpose

    bacterial protein expression of TIA1

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    TIA1 (TIA1 Human)
    

    Tags

    6xHIs-thrombin and T7

    Expression

    Bacterial

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. BCJJ345

    Plasmid

    #117504

    Purpose

    YAO:Cas9:E9 Golden Gate Level 1 Position 2 reverse

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:tagt:YAO:Cas9-3(intron):E9:ttgc:BpiI
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtYAO

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP-N1-IL6

    Plasmid

    #111933

    Purpose

    Expresses human interleukin-6 (IL6) fused to GFP in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

    Insert

    Interleukin 6 (IL6 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Codon optimized for expression in human cells

    Promoter

    CMV

    Available Since

    July 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ131-tRNA2.0

    Plasmid

    #158393

    Purpose

    Golden Gate entry vector to express the 1st gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    Without promoter

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBiFC-VC155

    Plasmid

    #22011

    Purpose

    Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

    Insert

    VC155
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Changed Alanine 206 to Lysine

    Available Since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBiFC-VC155

    Plasmid

    #22011

    Purpose

    Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

    Insert

    VC155
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Changed Alanine 206 to Lysine

    Available Since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSNAPf-C1

    Plasmid

    #58186

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    SNAP tag

    Expression

    Mammalian

    Available Since

    Jan. 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge