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Optimized axolotl (Ambystoma mexicanum) husbandry, breeding, metamorphosis, transgenesis and tamoxifen-mediated recombination.
Khattak S, Murawala P, Andreas H, Kappert V, Schuez M, Sandoval-Guzman T, Crawford K, Tanaka EM
Nat Protoc. 2014 Mar;9(3):529-40. doi: 10.1038/nprot.2014.040. Epub 2014 Feb 6.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
51817pBSII-SK-mTol2-MCSTo generate transgenic axolotls using Tol2 or SceI mediated transgenics
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51818T7-TPaseFor in vitro transcription of Tol2 transposase using T7 Promoter
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