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Gang Bao Lab Plasmids


The Gang Bao Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Bao Lab Golden Gate plasmids (unpublished)
Trans-spliced Cas9 allows cleavage of HBB and CCR5 genes in human cells using compact expression cassettes. Fine EJ, Appleton CM, White DE, Brown MT, Deshmukh H, Kemp ML, Bao G Sci Rep. 2015 Jul 1;5:10777. doi: 10.1038/srep10777.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
35188pTALE64GTAL Effector-VP64-T2A-eGFP (Other)Mammalian Expression, TALEN Bao Lab Golden Gate plasmids (unpublished) Golden Gate Compatible TALE Transcription Factor using VP64 Domain with T2A-GFP addition pcDNA3.1 Add to Cart
35189pTALE64Transcription Activator Like Effector Transcription Factor (Other)Mammalian Expression, TALEN Bao Lab Golden Gate plasmids (unpublished) Golden Gate Compatible TALE Transcription Factor using VP64 Domain pcDNA3.1 Add to Cart
40786pET-HIS-SangamoTranscription Activator Like Effector Nuclease (Other)Bacterial Expression, TALEN Bao Lab Golden Gate plasmids (unpublished) for e. coli expression, Golden Gate Compatible TALEN incorporated with N Terminal 6xHIS Tag, Codon Optimized FokI pET-28b Add to Cart
40787pET-Sangamo-HISTranscription Activator Like Effector Nuclease (Other)Bacterial Expression, TALEN Bao Lab Golden Gate plasmids (unpublished) for e. coli expression, Golden Gate Compatible TALEN incorporated with C Terminal 6xHIS Tag, Codon Optimized FokI pET-28b Add to Cart
40788TALEN_C63Transcription Activator Like Effector Nuclease (Other)Mammalian Expression, TALEN Bao Lab Golden Gate plasmids (unpublished) Golden Gate Compatible TALEN Construct for site specific modification of genome, +63 C Terminus, Codon Optimized FokI pcDNA3.1 Add to Cart
47974TALEN_C18Transcription Activator Like Effector Nuclease (Other)Mammalian Expression, TALEN Bao Lab Golden Gate plasmids (unpublished) Golden Gate Compatible TALEN Construct site specific modification of genome, shortened C Terminus, Codon Optimized FokI pcDNA3.1 Add to Cart
58693N-Terminal Split Cas9 with GyrA inteinHumanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein (Homo sapiens)Mammalian Expression, AAV, CRISPRTrans-spliced Cas9 allows cleavage of HBB and CCR5 genes in human cells using compact expression cassettes. Sci Rep. 2015 Jul 1;5:10777. doi: 10.1038/srep10777. Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production Plasmid 42230: pX330-U6-Chimeric_BB-CBh-hSpCas9 Add to Cart
58694C-Terminal Split Cas9 with GyrA inteinHumanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein (Homo sapiens)Mammalian Expression, AAV, CRISPRTrans-spliced Cas9 allows cleavage of HBB and CCR5 genes in human cells using compact expression cassettes. Sci Rep. 2015 Jul 1;5:10777. doi: 10.1038/srep10777. Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production Plasmid 42230: pX330-U6-Chimeric_BB-CBh-hSpCas9 Add to Cart
58695N-Terminal Split Cas9 D10A Nickase with GyrA inteinD10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein (Homo sapiens)Mammalian Expression, AAV, CRISPRTrans-spliced Cas9 allows cleavage of HBB and CCR5 genes in human cells using compact expression cassettes. Sci Rep. 2015 Jul 1;5:10777. doi: 10.1038/srep10777. Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production Plasmid 42235: pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A) Add to Cart