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pLenti-EF-FH-TAZ S89A-ires-blast
(Plasmid #52084)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52084 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLenti-EF-ires-blast
  • Modifications to backbone
    pLenti-EF-FH-TAZ S89A was constructed by subcloning NheI/SalI fragment from pCIneoFH-TAZ S89A into pLenti-EF-ires-blast vector. TAZ insert was amplified by PCR, cut with EcoRI and XhoI and ligated into EcoRI and Sall sites of pLenti-EF-ires-blast vector.
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TAZ S89A
  • Alt name
    Transcriptional co-activator with PDZ-binding motif
  • Alt name
    WWTR1
  • Species
    H. sapiens (human)
  • Mutation
    S89A
  • Entrez Gene
    WWTR1 (a.k.a. TAZ)
  • Promoter EF1a
  • Tags / Fusion Proteins
    • FLAG (N terminal on backbone)
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer EF1a-F
  • 3′ sequencing primer pBABE-3
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that TAZ has a Sall site present within the coding region.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-EF-FH-TAZ S89A-ires-blast was a gift from Yutaka Hata (Addgene plasmid # 52084 ; http://n2t.net/addgene:52084 ; RRID:Addgene_52084)
  • For your References section:

    Screening with a novel cell-based assay for TAZ activators identifies a compound that enhances myogenesis in C2C12 cells and facilitates muscle repair in the muscle injury model. Yang Z, Nakagawa K, Sarkar A, Maruyama J, Iwasa H, Bao Y, Ishigami-Yuasa M, Ito S, Kagechika H, Hata S, Nishina H, Abe S, Kitagawa M, Hata Y. Mol Cell Biol. 2014 Feb 18. 10.1128/MCB.01346-13 PubMed 24550007