|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||52085||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Modifications to backboneEmGFP and LATS2/1 KD were inserted into pLenti-EF/V5-DEST from pcDNA 6.2-GW/miR vector.
Vector typeMammalian Expression, Lentiviral, RNAi
Growth in Bacteria
- Cloning method Gateway Cloning
- 5′ sequencing primer EF1a-F
- 3′ sequencing primer pBABE-3 (Common Sequencing Primers)
Co-cistronic expression of LATS2/1 pre-miRNA with EmGFP as reporter.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti-EmGFP-LATS2/1 KD was a gift from Yutaka Hata (Addgene plasmid # 52085 ; http://n2t.net/addgene:52085 ; RRID:Addgene_52085)
For your References section:Screening with a novel cell-based assay for TAZ activators identifies a compound that enhances myogenesis in C2C12 cells and facilitates muscle repair in the muscle injury model. Yang Z, Nakagawa K, Sarkar A, Maruyama J, Iwasa H, Bao Y, Ishigami-Yuasa M, Ito S, Kagechika H, Hata S, Nishina H, Abe S, Kitagawa M, Hata Y. Mol Cell Biol. 2014 Feb 18. 10.1128/MCB.01346-13 PubMed 24550007