232,579 results

1. pmTurquoise2-Mito

   Plasmid

   #36208

   Purpose

   In vivo visualization of the mitochondria (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   MTS (COX8A Human)
   

   Tags

   mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmTurquoise2-Mito

   Plasmid

   #36208

   Purpose

   In vivo visualization of the mitochondria (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   MTS (COX8A Human)
   

   Tags

   mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pX459V2.0-HypaCas9

   Plasmid

   #108294

   Purpose

   pX459 V2.0 (Plasmid #62988) with the N692A, M694A, Q695A, and H698A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 puro Nodamura B2

   Plasmid

   #17228

   Depositor

   Christopher Sullivan

   Article

   Sullivan et al J Virol. 2005 Jun . 79(12):7371-9.

   Insert

   Nodamura B2 (NoVsRNA1gp2 Nodamura Virus)
   

   Expression

   Mammalian

   Mutation

   2 silent point mutations

   Available Since

   June 22, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pcDNA3.1 puro Nodamura B2

   Plasmid

   #17228

   Depositor

   Christopher Sullivan

   Article

   Sullivan et al J Virol. 2005 Jun . 79(12):7371-9.

   Insert

   Nodamura B2 (NoVsRNA1gp2 Nodamura Virus)
   

   Expression

   Mammalian

   Mutation

   2 silent point mutations

   Available Since

   June 22, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pENTR221 UBR5

   Plasmid

   #81062

   Purpose

   Gateway entry vector encoding full length UBR5

   Depositor

   Darren Saunders

   Article

   Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

   Insert

   Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) (UBR5 Human)
   

   Use

   Cloning

   Mutation

   K503R

   Promoter

   N/A

   Available Since

   Jan. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR221 UBR5

   Plasmid

   #81062

   Purpose

   Gateway entry vector encoding full length UBR5

   Depositor

   Darren Saunders

   Article

   Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

   Insert

   Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) (UBR5 Human)
   

   Use

   Cloning

   Mutation

   K503R

   Promoter

   N/A

   Available Since

   Jan. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRU42

   Plasmid

   #167664

   Purpose

   ENTRY vector for gRNA2 cloning under pAtU3 promoter

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pAtU3
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-Kir2.1Mut-T2A-tdTomato

   Plasmid

   #60644

   Purpose

   Expresses Kir2.1Mut-T2A-tdTomato

   Depositor

   Massimo Scanziani

   Article

   Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

   Insert

   Kcnj2 (Kcnj2 Mouse)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   GYG to AAA (aa144-146)

   Available Since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-Kir2.1Mut-T2A-tdTomato

    Plasmid

    #60644

    Purpose

    Expresses Kir2.1Mut-T2A-tdTomato

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Insert

    Kcnj2 (Kcnj2 Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    GYG to AAA (aa144-146)

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti CMV Puro DEST (w118-1) SDC3

    Plasmid

    #107501

    Purpose

    Expresses SDC3, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    SDC3 (codon optimized) (SDC3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Codon optimized sequence

    Promoter

    CMV

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9

    Plasmid Kit

    #1000000171

    Purpose

    Vectors and DNA modules for assembly of CRISPR/Cas9 constructs for use in plants. The constructs are made with zCas9i, a high efficiency intron-optimized SpCas9-coding gene.

    Depositor

    Sylvestre Marillonnet, Johannes Stuttmann

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

13. CMV-hEAAT2

    Plasmid

    #32814

    Depositor

    Susan Amara

    Insert

    hEAAT2 (SLC1A2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-hEAAT2

    Plasmid

    #32814

    Depositor

    Susan Amara

    Insert

    hEAAT2 (SLC1A2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCry2PHR-mCh-LRP6c

    Plasmid

    #42960

    Purpose

    Expresses optogenetically controlled LRP6 (C-terminal domain)

    Depositor

    David Schaffer

    Article

    Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

    Insert

    Cry2PHR-mCherry-LRP6c
    

    Expression

    Mammalian

    Mutation

    LPR6 cytoplasmic domain only

    Promoter

    CMV

    Available Since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCry2PHR-mCh-LRP6c

    Plasmid

    #42960

    Purpose

    Expresses optogenetically controlled LRP6 (C-terminal domain)

    Depositor

    David Schaffer

    Article

    Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

    Insert

    Cry2PHR-mCherry-LRP6c
    

    Expression

    Mammalian

    Mutation

    LPR6 cytoplasmic domain only

    Promoter

    CMV

    Available Since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PH-Akt(R25C)-GFP

    Plasmid

    #51466

    Purpose

    Non-PIP3 binding control for PH-Btk-GFP

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

    Insert

    AKT (aa 1–164)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R25C

    Promoter

    CMV

    Available Since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

18. PH-Akt(R25C)-GFP

    Plasmid

    #51466

    Purpose

    Non-PIP3 binding control for PH-Btk-GFP

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

    Insert

    AKT (aa 1–164)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R25C

    Promoter

    CMV

    Available Since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pSCAR_sgRNA_hygro-tagBFP-lox2272

    Plasmid

    #162078

    Purpose

    3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Expression

    Mammalian

    Promoter

    U6, EF1a

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE (AAV8)

    Viral Prep

    #131004-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE (#131004). In addition to the viral particles, you will also receive purified pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE plasmid DNA. hSyn-driven expression of calcium sensor GCaMP6m and bicistronic ChRmine (red-shifted channelrhodopsin). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Promoter

    Syn

    Available Since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only