168,234 results

1. pYO1280

   Plasmid

   #235712

   Purpose

   Expression of FL human VPS34-EGFP in mammalian cells

   Depositor

   Roger Williams

   Article

   Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

   Insert

   VPS34 (PIK3C3 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB-T-PAF-Clu

   Plasmid

   #215380

   Purpose

   High expression of Clusterin for purification.

   Depositor

   Franz-Ulrich Hartl

   Insert

   CLU (CLU Human)
   

   Expression

   Mammalian

   Promoter

   tetO

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. PAC_E83_LOV_eGFP

   Plasmid

   #231574

   Purpose

   This plasmid mediates puromycin resistance in the dark, resulting in puro mediated cell death upon illumination with blue light

   Depositor

   Dominik Niopek

   Article

   Wolf et al bioRxiv 2024.12.04.626757

   Insert

   PAC-P2A-GFP carrying an insertion of AsLOV2 behind E83
   

   Expression

   Mammalian

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYFJ84

   Plasmid

   #227644

   Purpose

   Vibrio harveyi acyl-ACP synthetase

   Depositor

   John Cronan

   Article

   Jiang et al Biochemistry. 2006 Aug 22;45(33):10008-1

   Insert

   Acyl-Acyl carrier protein synthetase
   

   Tags

   10xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmEGFP-N1

   Plasmid

   #217486

   Purpose

   Expression of monomeric Enhanced Green Fluorescent Protein

   Depositor

   Bertrand Collet

   Article

   Chaumont et al BMC Genomics. 2024 Jun 29;25(1):650. doi

   Insert

   mEGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDF-Leup

   Plasmid

   #213154

   Purpose

   Expresses the leupeptin protease inhibitor pathway for bolstered production of heterologously expressed proteins.

   Depositor

   Jason Crawford

   Article

   Li et al J Nat Prod. 2021 Oct 22;84(10):2692-2699

   Insert

   Leupeptin Protease Inhibitor Pathway
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3-SREBP-1 S1P and S2P mutations (U124AFG200-5)

   Plasmid

   #210767

   Purpose

   Mammalian expression of SREBP-1 (isoform 1) with mutations in residues reported to be critical for S1P and S2P cleavage.

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SREBP-1 (SREBF1 Human)
   

   Tags

   E-tag and T7x2

   Expression

   Mammalian

   Mutation

   DRSR 514-517 to AS; 557 R to A; multiple silent m…

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBiT3.1-N_RBBP4:1-425

   Plasmid

   #211037

   Purpose

   Mammalian expression of full-length human RBBP4. N-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   RBBP4:M1-S425 (RBBP4 Human)
   

   Tags

   MVSGWRLFKKISGSSGGSSG

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available Since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQE80l BCB

   Plasmid

   #196472

   Purpose

   Protein expression of SpyCatcher-ELP-CarHC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher

   Depositor

   Fei Sun

   Article

   Wang et al Proc Natl Acad Sci U S A. 2017 Jun 6;114

   Insert

   BCB
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP-SPN_ARR Q37A/R38A/N52R (Shank3)

    Plasmid

    #175256

    Purpose

    SPN-ARR fragment with structure opening N52R and actin binding site Q37A/R38A mutations in EGFP backbone for mammalian expression

    Depositor

    Johanna Ivaska

    Article

    Salomaa et al Curr Biol. 2021 Sep 29. pii: S0960-9822(

    Insert

    SPN-ARR fragment with Q37A/R38A/N52R mutations from SHANK3
    

    Expression

    Mammalian

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFA6a-kanMX6-P3nmt1-mECitrine

    Plasmid

    #105151

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

    Insert

    kanMX6-P3nmt1-mECitrine
    

    Tags

    3nmt1-mECitrine

    Expression

    Bacterial and Yeast

    Mutation

    F64L, Q69M, A206K

    Promoter

    3nmt1

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA6a-kanMX6-P81nmt1-mECitrine

    Plasmid

    #105153

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Davidson et al PLoS One. 2016 Nov 29;11(11):e0167043. d

    Insert

    kanMX6-P81nmt1-mECitrine
    

    Tags

    81nmt1-mECitrine

    Expression

    Bacterial and Yeast

    Mutation

    F64L, Q69M, A206K

    Promoter

    81nmt1

    Available Since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFA6a-kanMX6-P41nmt1-mECitrine

    Plasmid

    #105152

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Wang et al J Cell Biol. 2014 May 12;205(3):357-75.

    Insert

    kanMX6-P41nmt1-mECitrine
    

    Tags

    41nmt1-mECitrine

    Expression

    Bacterial and Yeast

    Mutation

    F64L, Q69M, A206K

    Promoter

    41nmt1

    Available Since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. jk68

    Plasmid

    #87020

    Purpose

    genomic Sindbis virus plasmid for use in SYNseq PLA screen

    Depositor

    Anthony Zador

    Article

    Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

    Inserts

    SNAP-Nlg1AB-4xλN(i)
    mCherry
    

    Use

    Synthetic Biology; Sindbis virus genomic plasmid

    Expression

    Mammalian

    Available Since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBP-lacZ

    Plasmid

    #72948

    Purpose

    Entry vector for Bioparts - Oligo anneal or PCR with internal BsaI sites at 5' and 3' ends using NdeI/SphI cloning

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 0 - Bioparts
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti6/V5-DEST-NCAPH

    Plasmid

    #31214

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    NCAPH (NCAPH Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDG02583

    Plasmid

    #104129

    Purpose

    Optimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    NEDD8-specific protease 1 (NEDP1)
    

    Tags

    14x Histidine tag, Maltose binding protein, and S…

    Expression

    Bacterial

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pH2B_mCherry_IRES_neo3

    Plasmid

    #21044

    Depositor

    Daniel Gerlich

    Article

    Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

    Insert

    pH2B
    

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CAG-SomaGCaMP6f2 (AAV8)

    Viral Prep

    #158757-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-CAG-SomaGCaMP6f2 (#158757). In addition to the viral particles, you will also receive purified pAAV-CAG-SomaGCaMP6f2 plasmid DNA. CAG-driven expression of soma-targeting GCaMP6f2. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Article

    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

    Promoter

    CAG promoter

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

20. pHLSec2-FN-YPet

    Plasmid

    #65421

    Purpose

    Expresses in mammalian cells human plasma fibronectin with YPet inserted between FNIII domains 6 and 7

    Depositor

    Harold Erickson

    Article

    Ohashi et al J Biol Chem. 2011 Nov 11;286(45):39188-9

    Insert

    human plasma fibronectin with YPet inserted between FNIII domains 6 and 7 (FN1 Human)
    

    Tags

    YPet

    Expression

    Mammalian

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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