Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pRR-PR-5Z
(Plasmid #23057)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 23057 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRW95-3
  • Backbone manufacturer
    PMID: 8800671
  • Backbone size w/o insert (bp) 8700
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PR
  • Alt name
    progesterone receptor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3000
  • Entrez Gene
    PGR (a.k.a. NR3C3, PR)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SnaBI (destroyed during cloning)
  • 3′ cloning site SnaBI (destroyed during cloning)
  • 5′ sequencing primer GAL1
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The cDNA used to clone this plasmid was obtained from K. Horwitz

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRR-PR-5Z was a gift from Charles Miller (Addgene plasmid # 23057 ; http://n2t.net/addgene:23057 ; RRID:Addgene_23057)
  • For your References section:

    Single plasmids expressing human steroid hormone receptors and a reporter gene for use in yeast signaling assays. Miller CA, Tan X, Wilson M, Bhattacharyya S, Ludwig S. Plasmid. 2009 Dec 3. ():. 10.1016/j.plasmid.2009.11.003 PubMed 19962400