|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||23058||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerPMID: 8800671
- Backbone size w/o insert (bp) 8700
Vector typeYeast Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameandrogen receptor
SpeciesH. sapiens (human)
Insert Size (bp)3000
Entrez GeneAR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
- Cloning method Restriction Enzyme
- 5′ cloning site SnaBI (destroyed during cloning)
- 3′ cloning site SnaBI (destroyed during cloning)
- 5′ sequencing primer GPDpro-F (Common Sequencing Primers)
Amino acid G8 is missing, BLAST shows different number of Qs in sequence.
Please note that this construct was tested by the depositing lab. Dr Miller transformed yeast strain W303a with Addgene's plasmid and with his stock of the same plasmid. He also streaked out yeast that were previously transformed with this plasmid and validated for signaling of the lacZ reporter gene in response to testosterone.
The plasmids transformed well and conferred reporter gene signaling in the transformants when exposed to 25 nM testosterone. This bioassay indicates that the human androgen receptor DNA sequence produces a functional protein.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRR-AR-5Z was a gift from Charles Miller (Addgene plasmid # 23058 ; http://n2t.net/addgene:23058 ; RRID:Addgene_23058)
For your References section:Single plasmids expressing human steroid hormone receptors and a reporter gene for use in yeast signaling assays. Miller CA, Tan X, Wilson M, Bhattacharyya S, Ludwig S. Plasmid. 2009 Dec 3. ():. 10.1016/j.plasmid.2009.11.003 PubMed 19962400
Map uploaded by the depositor.