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pRR-AR-5Z
(Plasmid #23058)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 23058 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRW95-3
  • Backbone manufacturer
    PMID: 8800671
  • Backbone size w/o insert (bp) 8700
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AR
  • Alt name
    androgen receptor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3000
  • Entrez Gene
    AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SnaBI (destroyed during cloning)
  • 3′ cloning site SnaBI (destroyed during cloning)
  • 5′ sequencing primer GPDpro-F
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The cDNA source for this plasmid was obtained from Aaron Caplan.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

Amino acid G8 is missing, BLAST shows different number of Qs in sequence.

Please note that this construct was tested by the depositing lab. Dr Miller transformed yeast strain W303a with Addgene's plasmid and with his stock of the same plasmid. He also streaked out yeast that were previously transformed with this plasmid and validated for signaling of the lacZ reporter gene in response to testosterone.

The plasmids transformed well and conferred reporter gene signaling in the transformants when exposed to 25 nM testosterone. This bioassay indicates that the human androgen receptor DNA sequence produces a functional protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRR-AR-5Z was a gift from Charles Miller (Addgene plasmid # 23058 ; http://n2t.net/addgene:23058 ; RRID:Addgene_23058)
  • For your References section:

    Single plasmids expressing human steroid hormone receptors and a reporter gene for use in yeast signaling assays. Miller CA, Tan X, Wilson M, Bhattacharyya S, Ludwig S. Plasmid. 2009 Dec 3. ():. 10.1016/j.plasmid.2009.11.003 PubMed 19962400