|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||23061||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerPMID: 8800671
- Backbone size w/o insert (bp) 8700
Vector typeYeast Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameestrogen receptor alpha
SpeciesH. sapiens (human)
Insert Size (bp)3000
Entrez GeneESR1 (a.k.a. ER, ESR, ESRA, ESTRR, Era, NR3A1)
- Cloning method Restriction Enzyme
- 5′ cloning site SnaBI (destroyed during cloning)
- 3′ cloning site SnaBI (destroyed during cloning)
- 5′ sequencing primer GAL1 (Common Sequencing Primers)
There is a M517T mutation in the ESR1 sequence. The mutation should not have any effect on protein function and this construct worked the same when assessed in yeast signaling assays and estrogen signaling.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRR-ERalpha-5Z was a gift from Charles Miller (Addgene plasmid # 23061 ; http://n2t.net/addgene:23061 ; RRID:Addgene_23061)
For your References section:Single plasmids expressing human steroid hormone receptors and a reporter gene for use in yeast signaling assays. Miller CA, Tan X, Wilson M, Bhattacharyya S, Ludwig S. Plasmid. 2009 Dec 3. ():. 10.1016/j.plasmid.2009.11.003 PubMed 19962400
Map uploaded by the depositor.