164,296 results

1. pDY1513 AAVS1 sgRNA

   Plasmid

   #234832

   Purpose

   sgRNA for AAVS1 STITCHR insertion

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

   Insert

   AAVS1 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. makeTCR_Lentiviral_Backbone

   Plasmid

   #233943

   Purpose

   backbone for makeTCR assembly

   Depositor

   Michael Platten

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a (long)

   Available since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2]

   Plasmid

   #84446

   Purpose

   AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CAG promoter, in floxed/reversed (Cre-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Has Service

   AAV1, AAV5, and AAV8

   Insert

   Jaws-KGC-tdTomato-ER2
   

   Use

   AAV

   Tags

   ER2, KGC, and tdTomato

   Expression

   Mammalian

   Mutation

   K200R W214F

   Promoter

   CAG

   Available since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti_gRNA -hMLHdn-Puro

   Plasmid

   #191105

   Purpose

   Backbone for lentiviral pegRNA library construction with hMLH1dn and Puro marker

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   hMLH1dn
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   I31 silent mutation (ATA to ATC) to remove early …

   Promoter

   EF-1a

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJQ200SK

   Plasmid

   #78497

   Purpose

   Suicide vector for gene replacement

   Depositor

   Michael Hynes

   Article

   Quandt et al Gene. 1993 May 15;127(1):15-21.

   Type

   Empty backbone

   Use

   Suicide plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHLSec2-FN-YPet

   Plasmid

   #65421

   Purpose

   Expresses in mammalian cells human plasma fibronectin with YPet inserted between FNIII domains 6 and 7

   Depositor

   Harold Erickson

   Article

   Ohashi et al J Biol Chem. 2011 Nov 11;286(45):39188-9

   Insert

   human plasma fibronectin with YPet inserted between FNIII domains 6 and 7 (FN1 Human)
   

   Use

   Tags

   YPet

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAV-CAG-oScarlet-WPRE-BC6-pA

   Plasmid

   #220770

   Purpose

   Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

   Depositor

   Vijay Samineni

   Article

   Yang et al Nat Commun. 2025 Jul 1;16(1):5557. doi:

   Insert

   oScarlert-WPRE-BC6
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. p4455 FLAG-hPLIC-2

   Plasmid

   #8661

   Depositor

   Peter Howley

   Article

   Kleijnen et al Mol Cell 2000 Aug;6(2):409-19.

   Insert

   hPLIC-2 (UBQLN2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 16, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. HDAC6 Flag

   Plasmid

   #13823

   Purpose

   Mammalian expression of human histone deacetylase 6 with flag tag

   Depositor

   Eric Verdin

   Article

   Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

   Insert

   HDAC6 (HDAC6 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5-(-2)AP-Ub

    Plasmid

    #232582

    Purpose

    Express Ubiquitin tagged with a modified Acceptor Peptide (AP)

    Depositor

    Sagar Bhogaraju

    Article

    Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

    Insert

    Ubiquitin
    

    Use

    Tags

    AP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET51b(+)_SNAP-tag2

    Plasmid

    #226512

    Purpose

    Bacterial cell expression of SNAP-tag2

    Depositor

    Kai Johnsson

    Article

    Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

    Insert

    SNAP-tag2
    

    Use

    Tags

    His-tag (10x) and Strep-Tag II,

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXs-c-Myc

    Plasmid

    #13375

    Purpose

    Retroviral expression of mouse c-Myc

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    myelocytomatosis oncogene (Myc Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMXs-mGCN2-FLAG-IP

    Plasmid

    #101794

    Purpose

    Retroviral vector encoding C-terminally FLAG tagged mouse GCN2

    Depositor

    Seiichi Oyadomari

    Article

    Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

    Insert

    Gcn2 (Eif2ak4 Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. EB3-tdTomato

    Plasmid

    #50708

    Purpose

    expresses EB3-tdTomato fusion in mammalian cells for labeling the growing tips of MTs

    Depositor

    Erik Dent

    Article

    Merriam et al J Neurosci. 2013 Oct 16;33(42):16471-82.

    Insert

    EB3 (MAPRE3 Human)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Ef1a-fDIO mCherry (AAV1)

    Viral Prep

    #114471-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-Ef1a-fDIO mCherry (#114471). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO mCherry plasmid DNA. Ef1a-driven, Flp recombinase-dependent expression of mCherry. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    EF1a

    Tags

    mCherry (Flp-dependent)

    Available since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

16. pN251

    Plasmid

    #188471

    Purpose

    Lentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)

    Depositor

    Le Cong

    Article

    Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

    Insert

    enCas12a-HF (gift from Dr. Keith Joung)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHCMV-MokolaG

    Plasmid

    #15811

    Depositor

    Miguel Sena-Esteves

    Article

    Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

    Insert

    Mokola virus glycoprotein (G) gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTagRFP658-N1

    Plasmid

    #178974

    Purpose

    Expresses TagRFP658 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    TagRFP658
    

    Use

    Tags

    no

    Expression

    Mammalian

    Mutation

    NO

    Promoter

    CMV

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. paavCAG-pre-mGRASP-mCerulean

    Plasmid

    #34910

    Purpose

    pre

    Depositor

    Jinhyun Kim

    Article

    Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

    Has Service

    AAV5

    Insert

    presynaptic mGRASP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Nov. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGP-AAV-syn-jGCaMP8s-WPRE (AAV Retrograde)

    Viral Prep

    #162374-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pGP-AAV-syn-jGCaMP8s-WPRE (#162374). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8s-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8s (more sensitive). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Promoter

    Syn

    Tags

    None

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only