166,671 results

1. pCMV-eNme2-C-ABE8e

   Plasmid

   #185667

   Purpose

   Expresses eNme2-C-ABE8e in mammalian cells

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   ecTadA(8e)-neNme2-C
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

   Promoter

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. XLone-PE2

   Plasmid

   #136463

   Purpose

   Expresses prime editing 2 (PE2) enzyme upon doxycycline treatment

   Depositor

   Xiaojun Lian

   Insert

   PE2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GS

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pThermoCas9_ctrl

   Plasmid

   #100981

   Purpose

   Expresses ThermoCas9 and its sgRNA module

   Depositor

   John van der Oost

   Article

   Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:

   Inserts

   Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
   ThermoCas9 single guide RNA expressing module
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   B. coagulans DSM 1 pta promoter and B. smithii xy…

   Available Since

   Nov. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CBh-mKate2-IRES-MCS (WT-WT)

   Plasmid

   #105921

   Purpose

   AAV-mediated gene expression from CBh promoter. Contains canonical left and right AAV2 ITRs

   Depositor

   Marcella Patrick

   Has Service

   AAV2

   Inserts

   mKate2
   IRES-Multiple cloning site
   

   Use

   AAV

   Tags

   Expression

   Mutation

   codon optimized

   Promoter

   CBh and same CBh promoter as mKate2

   Available Since

   Feb. 15, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pAAV-hSyn-GRAB_NEmut (AAV9)

   Viral Prep

   #123310-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-hSyn-GRAB_NEmut (#123310). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_NEmut plasmid DNA. hSyn-driven expression of GRAB-NEmut control norepinephrine sensor (does not respond to NE). These AAV preparations are suitable purity for injection into animals.

   Depositor

   Yulong Li

   Article

   Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

   Promoter

   Syn

   Tags

   None

   Available Since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

6. Cbh_v5 AAV-CBE C-terminal

   Plasmid

   #137176

   Purpose

   AAV genome: expresses the C-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   v5 AAV-CBE C-terminal; U6-protospacer
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Cbh

   Available Since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-(BbsI)_CBh-Cas9-T2A-mCherry

   Plasmid

   #64324

   Purpose

   Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available Since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMT006

   Plasmid

   #64761

   Purpose

   Recombination-Induced Tag Exchange (RITE) Cassette, 2xFLAG to an HA-6xHIS epitope tag, Hygro selection

   Depositor

   Fred van Leeuwen

   Article

   Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

   Insert

   RITE cassette
   

   Use

   Rite cassette

   Tags

   Expression

   Mutation

   Promoter

   none

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS130

   Plasmid

   #26290

   Depositor

   Tom Clandinin

   Article

   Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

   Insert

   phiC31 integrase (int Streptomyces phage phiC31)
   

   Use

   Drosophila transgenesis

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available Since

   Feb. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. Multiplex CRISPR/Cas9 Assembly System Kit

    Plasmid Kit

    #1000000055

    Purpose

    Golden Gate cloning assembly for construction of all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs with either Cas9 nuclease or Cas9 D10A nickase; see kit #1000000062 for FokI Accessory Pack

    Depositor

    Takashi Yamamoto

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pRRL.PPT.SFFV.HA.RUNX1C.IRES.eGFP

    Plasmid

    #181980

    Purpose

    Human RUNX1C gene overexpression

    Depositor

    Jan-Henning Klusmann

    Insert

    RUNX1C (human) (RUNX1 Human)
    

    Use

    Lentiviral

    Tags

    HA (Human influenza hemagglutinin)

    Expression

    Mutation

    Promoter

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EFS-SpCas9

    Plasmid

    #104588

    Purpose

    AAV vector expressing Myc-tagged SpCas9 under the EFS promoter

    Depositor

    Ryohei Yasuda

    Article

    Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

    Insert

    Streptococcus pyogenes Cas9
    

    Use

    AAV and CRISPR

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCoPURO

    Plasmid

    #17533

    Depositor

    Francis Castellino

    Article

    Iwaki et al Biotechniques. 2003 Sep . 35(3):482-4, 4

    Insert

    Puromycine Acetyl Transferase
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQE 80L Kan T7WT

    Plasmid

    #102790

    Purpose

    Expresses T7 RNA polymerase

    Depositor

    Andrew Ellington

    Article

    Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

    Insert

    T7 RNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 3, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pCambia1300-PacI

    Plasmid

    #44183

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-VSVP

    Plasmid

    #64088

    Purpose

    Expression vector for Vesicular Stomatitis Virus Indiana strain phosphoprotein gene

    Depositor

    Ian Wickersham

    Insert

    VSV phosphoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBAM1

    Plasmid

    #60487

    Purpose

    This plasmids is a modular mini-Tn5 vector that could be used to generate random mutagenesis libraries or to insert heterologous DNA into a target genome

    Depositor

    Víctor de Lorenzo

    Article

    Martinez-Garcia et al BMC Microbiol. 2011 Feb 22;11:38. doi: 1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. LARRYv2-T-Sapphire library 1

    Pooled Library

    #233213

    Purpose

    Barcoding pooled library

    Depositor

    Alejo Rodriguez-Fraticelli

    Article

    Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

    Species

    Synthetic

    Use

    Lentiviral

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

19. pX458-Dual-Guide-Donor-ShuttleOnly

    Plasmid

    #171098

    Purpose

    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    XbaI site added upstream of U6 promotor. GtoC mu…

    Promoter

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV1)

    Viral Prep

    #162381-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (#162381). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Promoter

    CAG

    Tags

    None

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only