161,601 results

1. pFastBac1-melHis10TEV

   Plasmid

   #159428

   Purpose

   empty pFastBac1 secretion vector (polyhedrin promoter) adds N-term melittin signal sequence & TEV-protease-removable His10; avoids unwanted residues in final protein by using BseRI restriction sites

   Depositor

   Debra Hansen

   Type

   Empty backbone

   Use

   Tags

   melittin signal sequence (removed in vivo by sign…

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmiRFP720-N1

   Plasmid

   #136560

   Purpose

   A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP720

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   miRFP720
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMDIAI

   Plasmid

   #51655

   Purpose

   E coli genetic modification

   Depositor

   Sheng Yang

   Article

   Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

   Insert

   apramycin antibiotic resistance gene
   

   Use

   Genetic modification

   Tags

   Expression

   Mutation

   Promoter

   unknown

   Available since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSIH1-puro-STAT3 shRNA

   Plasmid

   #26596

   Depositor

   Frank Sinicrope

   Article

   Huang et al Mol Cancer Ther. 2010 Mar . 9(3):742-50.

   Insert

   STAT3 shRNA (STAT3 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMU-NUCr

   Plasmid

   #61168

   Purpose

   Fluorescent reporter for nucleus NLS-mCherry-GUS expressed under AtUBQ10 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   NLS-mCherry-GUS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBa.Halo-LDLR

   Plasmid

   #162717

   Purpose

   Mammalian expression of human LDLR

   Depositor

   Marvin Bentley

   Article

   Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

   Insert

   LDLR (LDLR Human)
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Chicken Beta actin

   Available since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. ACER

   Pooled Library

   #226117

   Purpose

   The Advanced Catalogue of Epigenetic Regulators (ACER) library allows scientists to study how different epigenetic proteins influence gene activity and contribute to diseases.

   Depositor

   Rui Lu

   Article

   Zhou et al Blood. 2024 Aug 16:blood.2023023644. doi

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

8. pLenti6-CENP-A-mCherry

   Plasmid

   #89767

   Purpose

   Lentivirus destination vector for stable expression of mCherry-tagged kinetochore marker CENP-A

   Depositor

   Torsten Wittmann

   Article

   Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

   Insert

   CENP-A (CENPA Human)
   

   Use

   Lentiviral

   Tags

   3xHA and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEBTet_NADP-Snifit-cyto

   Plasmid

   #113917

   Purpose

   Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

   Insert

   NADP-Snifit
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV TetO2

   Available since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pL97

    Plasmid

    #78576

    Purpose

    Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains strong constitutive ermEp promoter

    Depositor

    Xuming Mao

    Article

    Sun et al Annals of Microbiology Dec 2012, Volume

    Type

    Empty backbone

    Use

    Streptomyces expression

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ermEp* promoter from S. erythraea

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. piggyBAC-5xTAPE-1-BlastR

    Plasmid

    #175808

    Purpose

    Encodes 5xTAPE-1 construct that can be integrated using piggyBAC system

    Depositor

    Jay Shendure

    Article

    Choi et al Nature. 2022 Jul 6. pii: 10.1038/s41586-

    Insert

    5xTAPE-1 construct
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. hCRISPRi-v2, genome-wide, top 5 sgRNAs/gene

    Pooled Library

    #83969

    Purpose

    Version 2 of the human CRISPRi genome wide library

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Use

    Lentiviral

    Available since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

13. 4xnrUAS:GFP

    Plasmid

    #61373

    Purpose

    4xnrUAS driving GFP

    Depositor

    Marnie Halpern

    Article

    Akitake et al Dev Biol. 2011 Apr 15;352(2):191-201. do

    Insert

    4xnr UAS:GFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pACBSR-hyg

    Plasmid

    #87830

    Purpose

    Expresses lambda-red system with hyg selection marker

    Depositor

    Pep Charusanti

    Article

    Huang et al BMC Res Notes. 2014 Jan 8;7:13. doi: 10.

    Insert

    hyg
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    None

    Promoter

    bla

    Available since

    Aug. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. PM-FRB-mRFP-T2A-FKBP-5-ptase

    Plasmid

    #40896

    Depositor

    Peter Varnai

    Article

    Toth et al J Cell Sci. 2012 May 1;125(Pt 9):2185-97

    Insert

    PM-FRB-mRFP-T2A-FKBP-5-ptase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmCherry-SopF

    Plasmid

    #135174

    Purpose

    Expresses an mCherry-SopF fusion in mammalian cells

    Depositor

    Leigh Knodler

    Article

    Lau et al PLoS Pathog. 2019 Jul 24;15(7):e1007959.

    Insert

    SopF (STM1239 Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. EC1000

    Bacterial Strain

    #71852

    Purpose

    RepA+ MC1000, KmR , carrying a single copy of the pWV01 repA gene in the glgB gene; host for pOR128-based plasmids

    Depositor

    Jan Kok, Todd Klaenhammer

    Article

    Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-

    Bacterial Resistance

    Kanamycin

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pHAGE-KRAS

    Plasmid

    #116755

    Purpose

    Lentiviral expression of KRAS

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    KRAS (KRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. UAS-mCherry

    Plasmid

    #135457

    Purpose

    Mammalian mCherry reporter to read out FLARE and SPARK activation

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    UAS

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. TFORF1440

    Plasmid

    #143808

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ARID4A (ARID4A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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