168,515 results

1. pT7-EGFP-C1-HsNXF-1_K

   Plasmid

   #146740

   Purpose

   Mammalian Expression of HsNxf-1

   Depositor

   Elisa Izaurralde

   Article

   Gatfield et al EMBO J. 2003 Aug 1. 22(15):3960-70.

   Insert

   HsNxf-1 (NXF1 Human)
   

   Expression

   Mammalian

   Mutation

   S144G relative to NM_006362.4

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCDH-puro-CMV-GC3AI

   Plasmid

   #78910

   Purpose

   Indicator of caspase-3-like protease activity

   Depositor

   Binghui Li

   Article

   Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

   Insert

   GC3AI
   

   Use

   Lentiviral

   Tags

   HA and Myc

   Mutation

   S320A, deletion of AA1-200

   Promoter

   CMV

   Available Since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. UAS:mCherry

   Plasmid

   #170994

   Purpose

   UAS:GAL4 repeat-containing promoter drives simple mCherry reporter gene. Blasticidin selectable.

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   UAS:mCherry
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   Synthetic 14x UAS:Gal4

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. C321.∆A.opt

   Bacterial Strain

   #87359

   Purpose

   This is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.

   Depositor

   George Church

   Article

   Kuznetsov et al Genome Biol. 2017 May 25;18(1):100. doi:

   Bacterial Resistance

   Gentamicin

   Species

   Synthetic

   Available Since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pLV hUbC-Cas9-T2A-GFP

   Plasmid

   #53190

   Purpose

   3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized Cas9 T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   hUbC

   Available Since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MSCVneo-HA-ER-Hoxb8

   Plasmid

   #222291

   Purpose

   The most commonly used plasmid for the generation of ER-Hoxb8 conditionally immortalized myeloid cell lines.

   Depositor

   Mark Kamps, David Sykes

   Article

   Wang et al Nat Methods. 2006 Apr;3(4):287-93.

   Insert

   ER-Hoxb8 (ESR1, Hoxb8 Human, Mouse)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   The estrogen receptor hormone binding domain cont…

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSIE1

   Plasmid

   #136355

   Purpose

   Selection by Inducible Effectors

   Depositor

   Andrew Goodman

   Article

   Bencivenga-Barry et al J Bacteriol. 2020 Jan 15;202(3). pii: JB

   Inserts

   Type VI effector bfe1
   Type VI effector bte1
   BT_4676
   tetR
   

   Use

   Unspecified

   Mutation

   ATG removed to add signal sequence before gene

   Promoter

   P1TDP-GHO23

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. spNW100

   Plasmid

   #173488

   Purpose

   Expresses spNW100 in Bl21

   Depositor

   Huan Bao

   Article

   Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

   Insert

   spNW100
   

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   P1049L- please see depositor comments

   Promoter

   T7

   Available Since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pIMAY

   Plasmid

   #68939

   Purpose

   E. coli/staphylococcal temperature-sensitive plasmid for allelic exchange

   Depositor

   Tim Foster

   Article

   Monk et al MBio. 2012 Mar 20;3(2). pii: e00277-11.

   Type

   Empty backbone

   Use

   Staphylococcal allelic exchange vector

   Available Since

   Sept. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA (AAV9)

    Viral Prep

    #20299-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA (#20299). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA plasmid DNA. EF1a-driven, Cre-dependent mCherry expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Tags

    mCherry (Cre-dependent)

    Available Since

    Sept. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

11. V48 ELP

    Plasmid

    #68395

    Purpose

    Elastin-like polypeptide (VPGxG) with 48 repeats of valine guest residue

    Depositor

    Andrew MacKay

    Article

    Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

    Insert

    V48 ELP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLifeAct-HyPer7

    Plasmid

    #136464

    Purpose

    Mammalian expression of F-actin targeted ultrasensitive hydrogen peroxide indicator HyPer7 fused with LifeAct peptide for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Tags

    LifeAct

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNIR-FbALFA

    Plasmid

    #184678

    Purpose

    NIR-Fb to ALFA-tag

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    NIR-FbALFA
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCS2+HyPer7-NLS

    Plasmid

    #136468

    Purpose

    Mammalian expression of nucleus targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Tags

    Triple nuclear localization signal of SV40 (simia…

    Expression

    Mammalian

    Promoter

    CMV, SP6

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pmVenus(L68V)-mTurquoise2

    Plasmid

    #60493

    Purpose

    Produces a fusion between mTurquoise2 and the FRET acceptor mVenus(L68V). It can be used as a positive control for FRET from mTurquoise2 to mVenus(L68V)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    SYFP2-mTurquoise2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 4xnrUAS:GFP

    Plasmid

    #61373

    Purpose

    4xnrUAS driving GFP

    Depositor

    Marnie Halpern

    Article

    Akitake et al Dev Biol. 2011 Apr 15;352(2):191-201. do

    Insert

    4xnr UAS:GFP
    

    Available Since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pH2B-emiRFP670

    Plasmid

    #136571

    Purpose

    Histone 2B labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    H2B-emiRFP670
    

    Tags

    emiRFP670

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFP-Ub

    Plasmid

    #11928

    Purpose

    Used for in vivo monitoring of ubiquitin localization.

    Depositor

    Nico Dantuma

    Article

    Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

    Insert

    Ubiquitin (UBC Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    June 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MZB209

    Plasmid

    #228492

    Purpose

    BirA encoded pACYC vector for co-expression with Avi-tagged constructs (Bacterial Expression)

    Depositor

    Richard Baker

    Insert

    BirA (birA )
    

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EF05J

    Plasmid

    #42941

    Depositor

    Eline Luning Prak

    Article

    Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

    Insert

    Negative control Functionally Dead Human L1 element (L1RE1 Human)
    

    Tags

    EGFP (opposite strand)

    Expression

    Mammalian

    Mutation

    ORF1 R261A, R262A

    Available Since

    April 25, 2013

    Availability

    Academic Institutions and Nonprofits only