304,601 results

1. mKeima-Red-N1

   Plasmid

   #54597

   Purpose

   Localization: N1 Cloning Vector, Excitation: 440, Emission: 620

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   mKeima-Red

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. HyPer7.2DAAO-NES

   Plasmid

   #168301

   Purpose

   Chemogenetic fluorescent H2O2 reporter targeted to the cytoplasm

   Depositor

   Thomas Michel

   Article

   Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

   Insert

   HyPer7.2DAAO-NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HyPer7.2DAAO-NES

   Plasmid

   #168301

   Purpose

   Chemogenetic fluorescent H2O2 reporter targeted to the cytoplasm

   Depositor

   Thomas Michel

   Article

   Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

   Insert

   HyPer7.2DAAO-NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CuO-LacI-NLS–SNAPf

   Plasmid

   #227368

   Purpose

   Lentiviral expression of LacI–SNAP

   Depositor

   David Pellman

   Article

   Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

   Insert

   LacI NLS SNAPf
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. C1(1-29)-miniTurbo-V5_pLX304

   Plasmid

   #107176

   Purpose

   expresses V5-tagged miniTurbo on the mammalian ER membrane, lentiviral vector

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   Available since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. C1(1-29)-miniTurbo-V5_pLX304

   Plasmid

   #107176

   Purpose

   expresses V5-tagged miniTurbo on the mammalian ER membrane, lentiviral vector

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   Available since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHage2-EF1a-TERT-IRES-Puro

   Plasmid

   #182590

   Purpose

   expresses TERT in mammalian cells

   Depositor

   Agnel Sfeir

   Article

   Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

   Insert

   hTERT (TERT Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHage2-EF1a-TERT-IRES-Puro

   Plasmid

   #182590

   Purpose

   expresses TERT in mammalian cells

   Depositor

   Agnel Sfeir

   Article

   Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

   Insert

   hTERT (TERT Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. plenti6-SLC7A11/xCT-V5

   Plasmid

   #170427

   Purpose

   Eepresses SLC7A11 (also named xCT) in mammalian cells

   Depositor

   Boyi Gan

   Article

   Koppula et al J Biol Chem. 2017 Aug 25;292(34):14240-1

   Insert

   solute carrier family 7 member 11 (SLC7A11 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. plenti6-SLC7A11/xCT-V5

    Plasmid

    #170427

    Purpose

    Eepresses SLC7A11 (also named xCT) in mammalian cells

    Depositor

    Boyi Gan

    Article

    Koppula et al J Biol Chem. 2017 Aug 25;292(34):14240-1

    Insert

    solute carrier family 7 member 11 (SLC7A11 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CMV-Mito4x-jRCaMP1b

    Plasmid

    #127873

    Purpose

    Red-shifted fluorescent reporter for mitochondrial matrix Ca2+

    Depositor

    Timothy Ryan

    Article

    Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

    Insert

    Red-shifted mitochondrial matrix Ca2+ indicator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CMV-Mito4x-jRCaMP1b

    Plasmid

    #127873

    Purpose

    Red-shifted fluorescent reporter for mitochondrial matrix Ca2+

    Depositor

    Timothy Ryan

    Article

    Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

    Insert

    Red-shifted mitochondrial matrix Ca2+ indicator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-Casp3-myc

    Plasmid

    #11813

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

    Insert

    Caspase 3 (CASP3 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Casp3-myc

    Plasmid

    #11813

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

    Insert

    Caspase 3 (CASP3 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SpyTag003-sfGFP

    Plasmid

    #133454

    Purpose

    Expresses SpyTag003-superfolder GFP in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyTag003-sfGFP
    

    Use

    Tags

    GSGSGS linker and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. SpyTag003-sfGFP

    Plasmid

    #133454

    Purpose

    Expresses SpyTag003-superfolder GFP in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyTag003-sfGFP
    

    Use

    Tags

    GSGSGS linker and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-nEF-Coff/Fon-ChR2-mCherry (AAV8)

    Viral Prep

    #137144-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-ChR2-mCherry (#137144). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-ChR2-mCherry plasmid DNA. nEF-driven, Flp-dependent expression of ChR2-mCherry for optogenetic activation (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    nEF

    Tags

    mCherry (Flp-dependent)

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

18. pLX307 P2A GFP Luciferase

    Plasmid

    #193674

    Purpose

    Constitutive lentiviral expression of Luciferase

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    Luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLX307 P2A GFP Luciferase

    Plasmid

    #193674

    Purpose

    Constitutive lentiviral expression of Luciferase

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    Luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCI-neo Fluc EGFP

    Plasmid

    #90170

    Purpose

    EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins

    Depositor

    Franz-Ulrich Hartl

    Article

    Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

    Insert

    FLuc EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge