165,975 results

1. Col1a-frt-hygro-pA

   Plasmid

   #20730

   Depositor

   Rudolf Jaenisch

   Article

   Beard et al Genesis. 2006 Jan . 44(1):23-8.

   Insert

   Col1A-frt-hygro-pA
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 24, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pOpen-P45

   Plasmid

   #165570

   Purpose

   Significantly improves maximum amplicon size and PCR yields for Family B polymerases like Pfu or Phusion. Comparable to ArchaeMaxx/PfuTurbo.

   Depositor

   Drew Endy, Jennifer Molloy, FreeGenes Project

   Insert

   P45
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 23, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. CP1041-ABE8e

   Plasmid

   #138493

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e)-nSpCas9 (CP1041)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LRG2.1

   Plasmid

   #108098

   Purpose

   Lentiviral expression plasmid of sgRNA with GFP

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter for sgRNA expression and EFS promoter…

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBAV1K-T5-gfp

   Plasmid

   #26702

   Depositor

   Ichiro Matsumura

   Article

   Bryksin et al PLoS One. 2010 . 5(10):e13244.

   Insert

   T5lacOlacOeGFP biobrick
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-TRKC-V5/HIS

   Plasmid

   #205615

   Purpose

   Expression of human TRKC receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   TRKC receptor tyrosine kinase (NTRK3 Human)
   

   Use

   Tags

   V5-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRBBm34

   Plasmid

   #48114

   Purpose

   Expression of the gfp gene under control of the native xylose inducible promoter of Bacillus megaterium

   Depositor

   Dieter Jahn

   Article

   Biedendieck et al J Biotechnol. 2007 Dec 1;132(4):426-30.

   Insert

   gfp
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA

   Plasmid

   #108871

   Purpose

   Expression of heat shock inducible Cas9-GFP and U6-driven 5 individual gRNAs for scGESTALT in zebrafish

   Depositor

   Alex Schier

   Article

   Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

   Inserts

   5xU6:sgRNA
   Cas9-t2A-GFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6 and hsp70

   Available Since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYZ205

   Plasmid

   #104586

   Purpose

   Express SNAP-6His-CBD

   Depositor

   Yinhua Zhang, New England Biolabs

   Insert

   SNAP-6His-CBD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 4, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pDESTmycDICER

    Plasmid

    #19873

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    dicer 1, ribonuclease type III (DICER1 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a-mH6-Cas12i1

    Plasmid

    #120882

    Purpose

    Expresses E. coli codon optimized Cas12i1 effector in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Cas12i1
    

    Use

    CRISPR

    Tags

    mH6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    lac

    Available Since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Mouse Metabolic Gene CRISPRa sgRNA library

    Pooled Library

    #187081

    Purpose

    Lentiviral CRISPRa library targeting mouse metabolic genes for activation of gene expression

    Depositor

    Kivanc Birsoy

    Article

    Unlu et al Mol Cell. 2022 Aug 4;82(15):2832-2843.e7

    Expression

    Mammalian

    Species

    Mus musculus

    Use

    Lentiviral

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

13. pRDA_221

    Plasmid

    #169142

    Purpose

    Constitutive expression of EGFP and sgRNAs targeted to EGFP for measurement of activity of AsCas12a\EnAsCas12a.

    Depositor

    John Doench, David Root

    Insert

    EGFP , EGFP sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pINDUCER20_mGFP

    Plasmid

    #190027

    Purpose

    Doxycycline-inducible lentiviral vector for expression of ORF with C-terminal mEGFP

    Depositor

    Andrew Tutt

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRSF-G1(4CNW)RS

    Plasmid

    #222871

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 4-Cyano-L-Tryptophan (4-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pETDuet-LahT150

    Plasmid

    #166785

    Purpose

    Cleavage of double glycine motif in peptides to remove the leader sequence

    Depositor

    Wilfred van der Donk

    Article

    Bobeica et al Elife. 2019 Jan 14;8. pii: 42305. doi: 1

    Insert

    LahT150
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRK-ATF3

    Plasmid

    #26115

    Depositor

    Yihong Ye

    Article

    Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

    Insert

    ATF3 (ATF3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-ApoE2

    Plasmid

    #197146

    Purpose

    Expresses ApoE2 in Mammalian cells

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    ApoE2 (APOE Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PB-TRE-GNL

    Plasmid

    #204499

    Purpose

    Expresses Glis1, Nanog and lin28a by tet-on promoter in mammalian cells

    Depositor

    Wataru Fujii

    Article

    Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

    Insert

    Glis1 (Glis1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mCherry-APP-mGFP

    Plasmid

    #196704

    Purpose

    Expresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cells

    Depositor

    Martino Calamai

    Article

    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

    Insert

    Amyloid Precursor Protein 695 isoform (APP Human)
    

    Use

    Tags

    mCherry and mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge