168,238 results

1. BFP-Rab5

   Plasmid

   #49147

   Purpose

   mammalian expression of BFP-Rab5 fusion

   Depositor

   Gia Voeltz

   Article

   Friedman et al Mol Biol Cell. 2013 Apr;24(7):1030-40. d

   Insert

   Rab5 (RAB5B Human)
   

   Tags

   BFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSR62

   Plasmid

   #125076

   Purpose

   Expression of ompR under PLtetO-1, output promoter PompF146

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

   Inserts

   ompR (ompR )
   sfgfp
   tetR
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-Camk2-NES-Twitch-GR

   Plasmid

   #173044

   Purpose

   TnC-based calcium sensor with green-red FRET pair ratiometric readout

   Depositor

   Yiyang Gong

   Article

   Zhang et al Commun Biol. 2021 Jul 29;4(1):924. doi:

   Has Service

   AAV2

   Insert

   NES-Twitch-GR
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CamK2

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-FLEX-TeLC-P2A-EYFP-WPRE

   Plasmid

   #135391

   Purpose

   Cre-dependent Tetanus toxin light chain construct for conditional silencing of neurons.

   Depositor

   Fan Wang

   Insert

   TeLC-P2A-EYFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSynapsin

   Available Since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPomyces-2

   Plasmid

   #61737

   Purpose

   Streptomyces expression of codon-optimized Cas9 and custom gRNA

   Depositor

   Huimin Zhao

   Article

   Cobb et al ACS Synth Biol. 2014 Dec 8.

   Inserts

   sSpCas9
   gRNA cassette
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   BbsI-flanked lacZ cassette inserted in place of s…

   Promoter

   gapdhp(EL) and rpsL(XC)-BbsI

   Available Since

   Jan. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAG-dLight1.3b (AAV9)

   Viral Prep

   #125560-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-CAG-dLight1.3b (#125560). In addition to the viral particles, you will also receive purified pAAV-CAG-dLight1.3b plasmid DNA. CAG-driven expression of dLight1.3b dopamine sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Lin Tian

   Article

   Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

   Promoter

   CAG

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

7. cyto-iATPSnFR1.0

   Plasmid

   #102550

   Purpose

   cytoplasmic ATP sensor for mammalian cells

   Depositor

   Baljit Khakh

   Article

   Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

   Insert

   iATPSnFR1.0
   

   Expression

   Mammalian

   Available Since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. HDAC3 Flag

   Plasmid

   #13819

   Purpose

   Mammalian expression of human histone deacetylase 3 with flag tag

   Depositor

   Eric Verdin

   Article

   Emiliani et al Proc Natl Acad Sci U S A. 1998 Mar 17. 9

   Insert

   HDAC3 (HDAC3 Human)
   

   Tags

   flag

   Expression

   Mammalian

   Available Since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. Red Glifon 300

   Plasmid

   #163115

   Purpose

   Red fluorescent protein-based glucose sensor for mammalian cells with higer affinity

   Depositor

   Takashi Tsuboi

   Article

   Mita et al Cell Chem Biol. 2021 Jun 29. pii: S2451-

   Insert

   Red Glifon 300
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. mEGFP-2xbiRhoBAST

    Plasmid

    #196349

    Purpose

    Expression of mEGFP mRNA with 2xbiRhoBAST in 3'UTR

    Depositor

    Murat Sunbul

    Article

    Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

    Insert

    mEGFP-2xbiRhoBAST
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Dual sgRNA CRISPRi Library

    Pooled Library

    #1000000192

    Depositor

    Jonathan Weissman

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

12. MCS-13X Linker-BioID2-HA

    Plasmid

    #80899

    Purpose

    To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.

    Depositor

    Kyle Roux

    Article

    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    HA and humanized A. aeolicus BioID2 with R40G

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIRES2-DsRed-MST-Kv2.1

    Plasmid

    #131707

    Purpose

    Expresses Kv2.1in mammalian cells

    Depositor

    Jennifer Kearney

    Article

    Jorge et al Proc Natl Acad Sci U S A. 2011 Mar 29;10

    Insert

    KCNB1 (KCNB1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. piggyBac-rtTA (4th_Gen)-sfGFP-IRES-NGN2-puro (VK_1122)

    Plasmid

    #209079

    Purpose

    Ngn2 mediated cortical neuron induction, along with sfGFP over-expression

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    sfGFP
    NGN2 (Neurog2 Mouse)
    

    Expression

    Mammalian

    Promoter

    TRE4G

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT7C-HSP90AB1

    Plasmid

    #177659

    Purpose

    Recombinant protein synthesis of human HSP90AB1

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    HSP90AB1 (HSP90AB1 Human)
    

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEE392

    Plasmid

    #149282

    Purpose

    T-DNA encoding TRV2 with mFT augmented gRNA targeting NbPDS3

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_NbPDS3sgRNA_mFT:tNos
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

17. pLenti Lifeact-EGFP BlastR

    Plasmid

    #84383

    Purpose

    Lentiviral expression of EGFP-tagged Lifeact with Blasticidin selection in cells including neurons

    Depositor

    Ghassan Mouneimne

    Article

    Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    EGFP

    Promoter

    CMV immediate early

    Available Since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. mEGFP-4xbiRhoBAST

    Plasmid

    #196350

    Purpose

    Expression of mEGFP mRNA with 4xbiRhoBAST in 3'UTR

    Depositor

    Murat Sunbul

    Article

    Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

    Insert

    mEGFP-4xbiRhoBAST
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRDB_345

    Plasmid

    #231113

    Purpose

    ABE activity-based selection cloning vector

    Depositor

    John Doench, David Root

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVX_DSB_Spectrum-V3

    Plasmid

    #192476

    Purpose

    Lentiviral DSB Spectrum V3 Reporter System

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    BFP1.2, mCherry, incompleteGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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