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Bright split red fluorescent proteins for the visualization of endogenous proteins and synapses.
Feng S, Varshney A, Coto Villa D, Modavi C, Kohler J, Farah F, Zhou S, Ali N, Muller JD, Van Hoven MK, Huang B
Commun Biol. 2019 Sep 17;2:344. doi: 10.1038/s42003-019-0589-x. eCollection 2019.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
117482pSFFV_sfCherry3C(1-10)Lentiviral vector for constitutive expression of sfCherry3C(1-10)
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117483pSFFV_sfCherry3V(1-10)Lentiviral vector for constitutive expression of sfCherry3V(1-10)
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117484pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10)Expresses tandem binder (SpyCatcher002-6aa-sfCherry2(1-10)) in mammalian cells
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117485pSFFV-SpyTag-sfCherry2(11)-TagBFPExpresses tandem tag (SpyTag002-GG-sfCherry2(11) fused to N-terminal of TagBFP) in mammalian cells
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117656pETDuet_sfCherry2(1-10)_sfCherry2(11)-SpyCatcherBacterial expression vector for split sfCherry3 screening. The “sfCherry2(1-10)” and “sfCherry2(11)-SpyCatcher002” are expressed from two different T7 promoters.
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Antibodies from Article