pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10)
(Plasmid #117484)

• Purpose: Expresses tandem binder (SpyCatcher002-6aa-sfCherry2(1-10)) in mammalian cells
• Depositing Lab: Bo Huang
• Publication: Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.1038/s42003-019-0589-x. eCollection 2019.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 6410
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SpyCatcher002-6aa-sfCherry2(1-10)
• Species: Synthetic
• Insert Size (bp): 990
• Promoter: CMV

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TTAAACTTAAGCTTGggcgccatggtaaccacc
• 3′ sequencing primer: CTGGACTAGTGGATCCTCAGTCCTCGTTGTGGCTGGTGAT

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/454041v1 for BioRxiv preprint