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pGL3-U6-sgRNA-EGFP

Plasmid

#107721

Purpose

For expression of sgRNA from the U6 promoter with EGFP expression simultaneously

Depositor

Xingxu Huang

Publication

Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

Type

Empty backbone

Use

CRISPR

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pC1-SypHer3s-dmito

Plasmid

#108119

Purpose

Mitochondrial ratiometric pH probe with enhanced brightness for mammalian cells expression. Probe is localized in mitochondrial matrix.

Depositor

Vsevolod Belousov

Publication

Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

Insert

SypHer3s-dmito

Tags

ratiometric YFP

Expression

Mammalian

Promoter

CMV

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCN-EF2132tet

Plasmid

#107191

Purpose

Expresses EF2132 recombinase for performing recombineering in S. aureus

Depositor

Steve Salipante

Publication

Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

Inserts

EF2132
tetR

Expression

Bacterial

Promoter

p23

Available since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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AAV-CMVc-Cas9

Plasmid

#106431

Purpose

Expresses SpCas9 from the CMVc promoter in AAV backbone

Depositor

Juan Belmonte

Publication

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Insert

Cas9

Use

AAV and CRISPR

Tags

HA-NLS and NLS

Expression

Mammalian

Promoter

CMVc

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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LcV2-Hygro

Plasmid

#91977

Purpose

LentiCRISPR v2 with Hygro cloned in place of Puro

Depositor

Joshua Mendell

Publication

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pGL4-ERSE1-luc2P-Hygro

Plasmid

#101789

Purpose

Luferase reporter plasmid containing three tandem repeats of the ER stress response element-I (ERSE-I)

Depositor

Seiichi Oyadomari

Publication

Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208

Insert

3xERSE-I

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

minimal TATA-box promoter with low basal activity

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCSC-ISL1-T2A-LHX3

Plasmid

#90215

Purpose

To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with NGN2, Sox11, FGF2 and two small molecules, forskolin and dorsomorphin.

Depositor

Chun-Li Zhang

Publication

Liu et al Cell Rep. 2016 Jan 5;14(1):115-28. doi:

Insert

ISL1-T2A-LHX3 (ISL1, LHX3 Human)

Use

Lentiviral

Promoter

CMV

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCMV-MbPylRS(DiZPK)

Plasmid

#91706

Purpose

express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interest

Depositor

P. Chen

Publication

Lin et al Angew Chem Int Ed Engl. 2013 Dec 23;52(5

Inserts

pylS
pylT

Expression

Mammalian

Mutation

L274A, C313S

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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6His-MBP-TEV-huLbCpf1

Plasmid

#90096

Purpose

Bacterial expression plasmid for protein purification

Depositor

Feng Zhang

Publication

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huLbCpf1

Tags

3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

Expression

Bacterial

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pT3-EF1aH N90-beta-catenin

Plasmid

#86499

Purpose

Mammalian expression of Myc tagged N90 beta-catenin

Depositor

Xin Chen

Publication

Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

Insert

beta-catenin (CTNNB1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Deletion of amino acids 1-90; constitutively acti…

Promoter

EF1a

Available since

Feb. 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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CSII-U6-gRNA-CBh-3xFLAG-PA-dCas9-P2A-Puro

Plasmid

#83306

Purpose

Lentivirus vector to express guideRNA and dCas9 with puro resistant gene

Depositor

Tohru Kimura

Publication

Murakami et al Nat Immunol. 2021 Mar;22(3):301-311. doi

Insert

sgRNA and dCas9 from pX330

Use

Lentiviral

Tags

FLAG and PA tags

Expression

Mammalian

Promoter

U6 for sgRNA and CBh for dCas9

Available since

Dec. 13, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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PR_GEF (2XPDZ-mCherry-Larg (DH)

Plasmid

#80407

Purpose

Recruitable mCherry tagged RhoA GEF LARG (DH domain). Expresses in mammalian cells

Depositor

Michael Glotzer

Publication

Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d

Insert

2XPDZ_mCherry_LARG DH (aa 766-997) (ARHGEF12 Human)

Tags

mCherry

Expression

Mammalian

Mutation

DH domain only (aa 766-997)

Promoter

CMV

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pGWB405

Plasmid

#74799

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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MIG-Luciferase-IRES-GFP

Plasmid

#75021

Purpose

Encodes the expression of Luciferase and GFP

Depositor

Saradhi Mallampati

Insert

Luciferase

Use

Luciferase and Retroviral

Available since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3-TfR-OVA

Plasmid

#64600

Purpose

Expression of chicken ovalbumin fused to the transmembrane domain of transferrin receptor as a model antigen for study of MHC II presentation

Depositor

Sandra Diebold, Martin Zenke

Publication

Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

Insert

Ovalbumin

Tags

Transmembrane domain of Transferrin Receptor (TfR…

Expression

Mammalian

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pU6b:sgRNA#3

Plasmid

#64247

Purpose

expresses sgRNA under U6b promoter

Depositor

Wenbiao Chen

Publication

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pHUE411

Plasmid

#62203

Purpose

CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Hyg resistance

Depositor

Qi-Jun Chen

Publication

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Inserts

gRNA scaffold
zCas9

Use

Plant binary vector

Tags

3x FLAG and NLS

Expression

Plant

Mutation

maize codon optimized

Promoter

OsU3 and Ubi

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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RA-NES

Plasmid

#61019

Purpose

ddRFP A-NES

Depositor

Robert Campbell

Publication

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddRFP A

Tags

HindIII site and NES sequence MNLVDLQKKLEELELDEQQ

Expression

Mammalian

Promoter

CMV

Available since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pQCXIN-TetR-mCherry

Plasmid

#59417

Purpose

retroviral construct expressing the Tet repressor (TetR) tagged with mCherry

Depositor

Tom Misteli

Publication

Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

Insert

Tet repressor protein (TetR)

Use

Retroviral

Tags

mCherry

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pL-CRISPR.SFFV.tRFP

Plasmid

#57826

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. SFFV Promoter driven

Depositor

Benjamin Ebert

Publication

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
SFFV
P2A-tRFP

Use

CRISPR and Lentiviral

Tags

FLAG

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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