165,995 results

1. pCMV-HyPer7-MEM

   Plasmid

   #136465

   Purpose

   Mammalian expression of cytosolic side of plasma membrane targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Use

   Tags

   Farnselation tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221_MFSD14A

   Plasmid

   #131869

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   MFSD14A (HIAT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 9, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. Tol2-ngn1-Trpa1b-2A-mCherry

   Plasmid

   #106425

   Purpose

   For expressing Trpa1b-2A-mCherry in zebrafish Rohon-Beard neurons.

   Depositor

   Randall Peterson

   Article

   Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

   Inserts

   cmlc2 promoter
   Trpa1b (trpa1b Zebrafish)
   

   Use

   Gateway

   Tags

   2A-mCherry

   Expression

   Mutation

   Promoter

   Available Since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

4. pSNA

   Plasmid

   #135192

   Purpose

   Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains ampicillin resistance cassette

   Depositor

   Hongqing Fang

   Article

   Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

   Insert

   I-SceI lamba Red
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   araBAD

   Available Since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. LZF42: Cre-off hSyn-QuasAr3-Citrine (FAS)

   Plasmid

   #104116

   Purpose

   synOptopatch construct

   Depositor

   Adam Cohen

   Article

   Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

   Insert

   QuasAr3-Citrine
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin I

   Available Since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCROPseq-VEX-optimized-scaffold

   Plasmid

   #203321

   Purpose

   Modified CROPseq vector for efficient CRISPR screens in hematopoiesis

   Depositor

   Vijay Sankaran

   Article

   Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

   Insert

   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHBS1500 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaNTD]

   Plasmid

   #133324

   Purpose

   To test the effect of sequence on TDP43 splicing activity

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of RRM in full-length TDP43

   Promoter

   Available Since

   Nov. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. WDR59 in pRK5

   Plasmid

   #154214

   Purpose

   Untagged WDR59 cDNA expression vector for transfections

   Depositor

   David Sabatini

   Article

   Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

   Insert

   WDR59 (WDR59 Human)
   

   Use

   Tags

   NONE

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJYS2.1-crtYf

   Plasmid

   #89077

   Purpose

   Constitutive transcription of crRNA of crtYF. CRISPR of C. glutamicum.

   Depositor

   Sheng Yang

   Article

   Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

   Insert

   crRNA of crtYF
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF1907

    Plasmid

    #141884

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TCF7L2 (TCF7L2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 FLAG-YY1

    Plasmid

    #104396

    Purpose

    FLAG-YY1

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Insert

    YY1
    

    Use

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-mH6-Cas12i2

    Plasmid

    #120883

    Purpose

    Expresses E. coli codon optimized Cas12i2 effector in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Cas12i2
    

    Use

    CRISPR

    Tags

    mH6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    lac

    Available Since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. LentiV_Cas9_Blast

    Plasmid

    #125592

    Purpose

    Lentiviral expression of spCas9 with blasticidin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS promoter

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHBS1502 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaCTD]

    Plasmid

    #133326

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of NTD in full-length TDP43

    Promoter

    Available Since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET His6 GST TEV LIC cloning vector (1G)

    Plasmid

    #29655

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, GST, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. murine TCR OTII-2A.pMIG II

    Plasmid

    #52112

    Purpose

    retroviral expression of both TCRalpha and TCRbeta using a single vector

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Protoc. 2006;1(1):406-17.

    Inserts

    T cell receptor alpha chain (Tcra Mouse)
    T cell receptor beta chain (Tcrb Mouse)
    

    Use

    Retroviral

    Tags

    P2A (from porcine teschovirus-1)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCasY1

    Plasmid

    #87687

    Purpose

    p15A plasmid expressing CasY1

    Depositor

    Jennifer Doudna

    Article

    Burstein et al Nature. 2016 Dec 22. doi: 10.1038/nature

    Insert

    CasY1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pnCasPA-BEC

    Plasmid

    #113349

    Purpose

    A cytidine deaminase-mediated base-editing plasmid in Pseudomonas species

    Depositor

    Quanjiang Ji

    Article

    Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-GFP-TFE3pA

    Plasmid

    #120201

    Purpose

    Human TFE3 poly(A) site reporter

    Depositor

    David Bartel

    Article

    Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

    Insert

    TFE3 poly(A) site
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBABE HA Chk2 WT

    Plasmid

    #41901

    Depositor

    Stephen Elledge

    Article

    Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

    Insert

    CHK2 (CHEK2 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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