168,238 results

1. pHR-UCOE-SFFV-Zim3-dCas9-P2A-mCherry

   Plasmid

   #188779

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   Zim3 KRAB-NLS fusion

   Promoter

   SFFV

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK-ATF3

   Plasmid

   #26115

   Depositor

   Yihong Ye

   Article

   Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

   Insert

   ATF3 (ATF3 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Dec. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2+8CmCherry

   Plasmid

   #34935

   Depositor

   Amro Hamdoun

   Article

   Gokirmak et al J Biol Chem. 2012 Nov 2.

   Type

   Empty backbone

   Use

   Sea urchin, zebrafish, xenopus

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   SP6

   Available Since

   Nov. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMVTNT-T7-KPNA6

   Plasmid

   #26682

   Depositor

   Bryce Paschal

   Article

   Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

   Insert

   Karyopherin Alpha 6 (KPNA6 Human)
   

   Tags

   T7

   Expression

   Mammalian

   Available Since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. Px461-Cas9n-Trp53-sgRNA-beta

   Plasmid

   #88847

   Purpose

   CRISPR KO of Trp53

   Depositor

   Joan Massague

   Article

   Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

   Insert

   Trp53 (Trp53 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

6. pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1

   Plasmid

   #183951

   Purpose

   Inducible lentiviral expression, TRE-control-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

   Depositor

   Eugene Yeo

   Article

   Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

   Insert

   APOBEC1 (Apobec1 Rat)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet ON

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pABpuro-BluF

   Plasmid

   #46824

   Purpose

   luciferase reporter for BmalI promoter

   Depositor

   Steven Brown

   Article

   Brown et al PLoS Biol. 2005 Oct;3(10):e338. Epub 200

   Insert

   Bmal1 (Bmal1 Mouse)
   

   Use

   Lentiviral and Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   Bmal1

   Available Since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLS-mP

   Plasmid

   #81225

   Purpose

   Lentivirus enhancer assay plasmid that contains a minimal promoter (mP) and a EGFP reporter gene.

   Depositor

   Nadav Ahituv

   Article

   Inoue et al Genome Res. 2016 Nov 9. pii: gr.212092.1

   Type

   Empty backbone

   Use

   Lentiviral

   Promoter

   Derived from pGL4.23

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKM512

   Plasmid

   #140192

   Purpose

   For curing of Orbit-integrated plasmids and associated drug marker

   Depositor

   Kenan Murphy

   Article

   Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

   Inserts

   zeocin resistance gene
   Bxb1gp47 (directionality factor)
   

   Expression

   Bacterial

   Mutation

   Cloned in gp47 directinality factor in place of R…

   Promoter

   EM7 and Ptet

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Px461-Cas9n-Trp53-sgRNA-alpha

    Plasmid

    #88846

    Purpose

    CRISPR KO of Trp53

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

11. H52-pUCM-hNGN2-inv

    Plasmid

    #110492

    Purpose

    Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology arm

    Depositor

    Michael Ward

    Inserts

    Neurogenin 2 (NEUROG2 Human)
    mcherry
    rtTA3G
    

    Use

    CRISPR, Cre/Lox, and TALEN

    Expression

    Mammalian

    Promoter

    CAG, EF-1 alpha, and TRE3G

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBabe K-Ras 12V

    Plasmid

    #12544

    Depositor

    Channing Der

    Article

    Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

    Insert

    K-Ras 12V (KRAS Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    12V constituitively activated

    Available Since

    Sept. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. murine TCR OTII-2A.pMIG II

    Plasmid

    #52112

    Purpose

    retroviral expression of both TCRalpha and TCRbeta using a single vector

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Protoc. 2006;1(1):406-17.

    Inserts

    T cell receptor alpha chain (Tcra Mouse)
    T cell receptor beta chain (Tcrb Mouse)
    

    Use

    Retroviral

    Tags

    P2A (from porcine teschovirus-1)

    Expression

    Mammalian

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-AKAR4-NES

    Plasmid

    #64727

    Purpose

    Cytosol-targeted, FRET-based PKA activity reporter.

    Depositor

    Jin Zhang

    Article

    Herbst et al Mol Cell Biol. 2011 Oct;31(19):4063-75.

    Insert

    AKAR4-NES
    

    Tags

    Cerulean, NES, and cpVenus[E172]

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLL5.0-MRLC-WT-GFP

    Plasmid

    #187258

    Purpose

    Lentiviral expression of myosin regulatory light chain-WT

    Depositor

    Alpha Yap

    Article

    Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

    Insert

    MRLC1 (MYL9 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Promoter

    5' LTR

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cavb3

    Plasmid

    #26574

    Depositor

    Diane Lipscombe

    Insert

    calcium channel beta subunit-III (Cacnb3 Rat)
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK5-myc-Rac1-T17N

    Plasmid

    #12984

    Depositor

    Gary Bokoch

    Insert

    Rac1 DN (RAC1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    T17N Dominant negative

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-HA-USP48

    Plasmid

    #22585

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP48 (USP48 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Dec. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUF-Cas9-pre-sgRNA

    Plasmid

    #137845

    Purpose

    Cas9 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

    Depositor

    Josh Beck

    Article

    Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CAG-iGluSnFR4f-NGR-WPRE

    Plasmid

    #234452

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f-NGR
    

    Use

    AAV

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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