PurposeExpression of dCas9 gene under the control of a weak Ptet promoter in the E. coli chromosome
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||115925||Bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Growth in Bacteria
Growth Strain(s)E. coli K12 MG1655
E. coli K12 MG1655 genotype: F- λ- ilvG- rfb-50 rph-1
Integration at lambda attB: pOSIP-KL-mCherry
Integration at primary 186 attB: pOSIP-CO-RBS-librarydCas9 (2-3)*
*Number in parentheses refers to the selected colony number.
mCherry quantifies dCas9 repression
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:LC-E75 was a gift from David Bikard (Addgene plasmid # 115925)
For your References section:A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9. Cui L, Vigouroux A, Rousset F, Varet H, Khanna V, Bikard D. Nat Commun. 2018 May 15;9(1):1912. doi: 10.1038/s41467-018-04209-5. 10.1038/s41467-018-04209-5 [pii] PubMed 29765036