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A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9.
Cui L, Vigouroux A, Rousset F, Varet H, Khanna V, Bikard D
Nat Commun. 2018 May 15;9(1):1912. doi: 10.1038/s41467-018-04209-5.
PubMed

Plasmids from Article

ID Plasmid Purpose  
114005psgRNAexpress sgRNA. ColE1, Kan
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114006psgRNAcexpress sgRNA, p15A, CRM
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115924LC-E18Expression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome
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115925LC-E75Expression of dCas9 gene under the control of a weak Ptet promoter in the E. coli chromosome
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115926AV04Expression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome
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115927psgRNA LibraryGenome-wide knockdown of E. coli genes
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116947pOSIP-KL-mcherryIntegrating mCherry into lambda attB in E. coli chromosome.
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