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Evolving accelerated amidation by SpyTag/SpyCatcher to analyze membrane dynamics.
Keeble AH, Banerjee A, Reddington SC, Ferla MP, Howarth M, Khairil Anuar INA
Angew Chem Int Ed Engl. 2017 Oct 10. doi: 10.1002/anie.201707623.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
102827pDEST14-SpyCatcher002Bacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteins
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102828pDEST14-Avitag-SpyCatcher002Bacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylation
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102829pDEST14-Cys-SpyCatcher002Bacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagents
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102830pDEST14-SpyCatcher002 EQBacterial expression of unreactive version of SpyCatcher002
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102831pET28a-SpyTag002-MBPBacterial expression of SpyTag002 fused to maltose binding protein
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102832pET28a-SpyTag002 DA-MBPBacterial expression of unreactive version of SpyTag002 fused to maltose binding protein
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Antibodies from Article