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pDEST14-SpyCatcher002 EQ
(Plasmid #102830)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 102830 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDEST14
  • Backbone manufacturer
    Thermo Fisher Scientific
  • Backbone size w/o insert (bp) 6422
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpyCatcher002 EQ
  • Species
    Synthetic
  • Insert Size (bp)
    435
  • Mutation
    E77Q mutation prevents isopeptide bond formation
  • Promoter T7
  • Tags / Fusion Proteins
    • His6 (N terminal on backbone)
    • TEV cleavage site (N terminal on insert)
    • GSSGS (C terminal on insert)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST14-SpyCatcher002 EQ was a gift from Mark Howarth (Addgene plasmid # 102830 ; http://n2t.net/addgene:102830 ; RRID:Addgene_102830)
  • For your References section:

    Evolving accelerated amidation by SpyTag/SpyCatcher to analyze membrane dynamics. Keeble AH, Banerjee A, Reddington SC, Ferla MP, Howarth M, Khairil Anuar INA. Angew Chem Int Ed Engl. 2017 Oct 10. doi: 10.1002/anie.201707623. 10.1002/anie.201707623 PubMed 29024296