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Showing: 5981 - 6000 of 242843 results

  5. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid
    #223065
    Purpose
    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    Use
    CRISPR
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Mutation
    Three regions of SpCas9 encode type IIS restricti…
    Promoter
    Dual T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223065-plasmid-map-sequence-id-447614

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  6. pAAV-syn-FLP-FRT-iGluSnFR4s-NGR-WPRE

    Plasmid
    #234453
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s-NGR
    Use
    AAV; Flp/frt
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234453-plasmid-map-sequence-id-465748

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  10. pFA6a-link-ymScarletI3-URA3

    Plasmid
    #224075
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with URA3 selection
    Depositor
    Bas Teusink
    Insert
    ymScarletI3
    Use
    Tags
    ymScarletI3
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    224075-plasmid-map-sequence-id-460510

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  11. pFA6a-link-ymScarletI3-Kan

    Plasmid
    #224077
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with Kan selection
    Depositor
    Bas Teusink
    Insert
    ymScarletI3
    Use
    Tags
    ymScarletI3
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    224077-plasmid-map-sequence-id-460512

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  13. pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPRE (AAV8)

    Viral Prep
    #183519-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPRE (#183519). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPRE plasmid DNA. CaMKIIa-driven expression of ChRmine-oScarlet-KV 2.1. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do
    Promoter
    CaMKII
    Tags
    None
    Available since
    Dec. 18, 2024
    Availability
    Academic Institutions and Nonprofits only

  17. pLenti-nSpCas9-APOBEC3A

    Plasmid
    #209040
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC3A is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    APOBEC3A-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209040-plasmid-map-sequence-id-413592

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  19. pBTK1050

    Plasmid
    #209540
    Purpose
    BTK Type 1 homology arm for Golden Gate assembly
    Depositor
    Jeffrey Barrick
    Article
    Lariviere et al bioRxiv 2023.09.19.558440
    Insert
    Upstream homology arm for recombination into the S. alvi wkB2 genome w/in the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    209540-plasmid-map-sequence-id-422358

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  20. pBTK1051

    Plasmid
    #209541
    Purpose
    BTK Type 5 homology arm for Golden Gate assembly
    Depositor
    Jeffrey Barrick
    Article
    Lariviere et al bioRxiv 2023.09.19.558440
    Insert
    Recombination into the S. alvi wkB2 genome within the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 2, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    209541-plasmid-map-sequence-id-421604

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Showing: 5981 - 6000 of 242843 results