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Fluorescence Lifetime Imaging of pH along the Secretory Pathway.
Linders PTA, Ioannidis M, Ter Beest M, van den Bogaart G
ACS Chem Biol. 2022 Jan 21;17(1):240-251. doi: 10.1021/acschembio.1c00907. Epub 2022 Jan 10.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
171716pRpHLuorin2-N1Ratiometric pHLuorin2 backbone (C terminal fusion)
171717ER-RpHLuorin2Luminal ER expression of ratiometric pHLuorin2
171718MGAT2-RpHLuorin2Cis-/medial-Golgi expression of ratiometric pHLuorin2
171719GalT-RpHLuorin2Trans-Golgi expression of ratiometric pHLuorin2
171720LAMP1-RpHLuorin2Lysosomal expression of ratiometric pHLuorin2
171721GPI-RpHLuorin2GPI-anchored RpHLuorin2 for plasma membrane expression (extracellular)
171722Str-KDEL_TNF-SBP-RpHLuorin2Synchronize trafficking of TNFa-RpHLuorin2
180227RpHLuorin2for bacterial expression of recombinant ratiometric pHluorin2

Antibodies from Article