ER-RpHLuorin2
(Plasmid #171717)

• Purpose: Luminal ER expression of ratiometric pHLuorin2
• Depositing Lab: Geert van den Bogaart
• Publication: Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251. doi: 10.1021/acschembio.1c00907. Epub 2022 Jan 10.

Backbone

• Vector backbone: pRpHLuorin2
• Backbone size w/o insert (bp): 4730
• Total vector size (bp): 4766
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ratiometric pHLuorin2 targeted to ER
• Species: Synthetic
• Promoter: CMV
• Tags / Fusion Proteins:
  • KDEL ER-retention signal (C terminal on insert)
  • Signal sequence of human calreticulin (first 18 residues) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: BsrGI (not destroyed)
• 5′ sequencing primer: pEGFP-cmv
• 3′ sequencing primer: pEGFP-all

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Ratiometric pHLuorin2 sequence synthesized by Genscript, original sequence from https://www.scirp.org/journal/paperinformation.aspx?paperid=5043