161,697 results

1. pcDNA3.1(+) EGFP-RalA(WT)

   Plasmid

   #224274

   Purpose

   Cell transfection and expression of RalA

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   RalA (RALA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CAG-LiLac

   Plasmid

   #184570

   Purpose

   Fluorescent reporter for lactate (with lifetime and intensity response); expresses in mammalian cells and can be packaged as AAV

   Depositor

   Gary Yellen

   Article

   Koveal et al Nat Commun. 2022 May 25;13(1):2919. doi:

   Insert

   LiLac fluorescent sensor of lactate
   

   Use

   AAV

   Tags

   His7

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-HIF1alpha P402A/P564A-pBabe-puro

   Plasmid

   #19005

   Depositor

   William Kaelin

   Article

   Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

   Insert

   Hypoxia inducible factor 1 alpha (HIF1A Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   cDNA changed amino acids: Proline 402 to Alanin…

   Promoter

   Available since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1_PA-Cre

   Plasmid

   #122960

   Purpose

   Photoactivatable Cre recombinase based on split Cre fragments and Magnet system (pMag and nMag)

   Depositor

   Moritoshi Sato

   Article

   Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

   Insert

   pcDNA3.1_PA-Cre
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMLS7-egfp

   Plasmid

   #112917

   Purpose

   Fluorescence microscopy

   Depositor

   Miguel Valvano

   Article

   Lefebre et al Appl Environ Microbiol. 2002 Dec;68(12):

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PS7 promoter and Ps7

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiTet-P2A-mCherry BlastR

   Plasmid

   #186743

   Purpose

   Dox inducible P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

   Depositor

   Richard Sherwood

   Article

   Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

   Insert

   P2A-mcherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. #1.4 pre-R-SynapShot-TagBFP

   Plasmid

   #214719

   Purpose

   Expresses red #1.4 pre-SynapShot and TagBFP

   Depositor

   Won Do Heo

   Article

   Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

   Insert

   #1.4 pre-R-SynapShot
   

   Use

   AAV

   Tags

   TagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. EB3-mNeonGreen

   Plasmid

   #191761

   Purpose

   Expression of mNeonGreen fused to EB3

   Depositor

   Daniel Gerlich

   Article

   Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

   Insert

   EB3-mNeonGreen (MAPRE3 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-TLR9-YFP

   Plasmid

   #13642

   Depositor

   Doug Golenbock

   Insert

   Toll-Like Receptor 9 (TLR9 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFH2.2_mCherry-MS2x12

    Plasmid

    #205521

    Purpose

    Express mCherry-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2 Notch1 Full Length-6MT

    Plasmid

    #41728

    Depositor

    Raphael Kopan

    Article

    Schroeter et al Nature. 1998 May 28;393(6683):382-6.

    Insert

    Notch-1 Full Length (Notch1 Mouse)
    

    Use

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    L1997V; Contains murine Notch-1 protein up to ami…

    Promoter

    Available since

    Dec. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV- κLight1.3

    Plasmid

    #201223

    Purpose

    Express kLight1.3 biosensor in mammalian cells

    Depositor

    Lin Tian

    Article

    Dong et al Nat Neurosci. 2024 Jul 15. doi: 10.1038/

    Insert

    κLight1.3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEvol-NnSULT1C1-cysDNCQ

    Plasmid

    #188983

    Purpose

    biosynthesize sulfotyrosine in E. coli.

    Depositor

    Han Xiao

    Article

    Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

    Inserts

    NnSULT1C1
    cysD
    cysN
    cysC
    cysQ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TFORF1438

    Plasmid

    #143807

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    EP400 (EP400 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Ef1a-vCreDIO hChR2(H134R)-EYFP

    Plasmid

    #55643

    Purpose

    vCre-Dependent ChR2-EYFP

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    hChR2(H134R)-EYFP
    

    Use

    AAV; Vcre-dependent chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETM6-G6-vioABE-4A6-vioC-3A2-vioD

    Plasmid

    #73440

    Purpose

    Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    PG6-vioABE-P4A6-vioC-P3A2-vioD
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PG6, P4A6, and P3A2 (orthogonal T7-lac variants)

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pClneoMyc-LATS2

    Plasmid

    #66852

    Purpose

    Express Myc-tagged LATS2 in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

    Insert

    LATS2 (LATS2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pU6-ACTA2_gRNA1-SpCas9-T2A-GFP

    Plasmid

    #126706

    Purpose

    to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequence

    Depositor

    Darrell Kotton

    Article

    Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

    Insert

    ACTA2_gRNA1-SpCas9-T2A-GF (ACTA2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. HA-p62

    Plasmid

    #28027

    Purpose

    Mammalian expression of HA tagged human p62

    Depositor

    Qing Zhong

    Article

    Fan et al Autophagy. 2010 Jul 22. 6(5):.

    Insert

    p62 (SQSTM1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Flag-HA-USP48

    Plasmid

    #22585

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP48 (USP48 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Dec. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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