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Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system.
Xie K, Minkenberg B, Yang Y
Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5. doi: 10.1073/pnas.1420294112. Epub 2015 Mar 2.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
63142pRGEB32Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.
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63143pGTRPCR template to construct polycistronic tRNA-gRNA
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63159pRGE32Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for transient expression in protoplast
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