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(Plasmid #63159)


Item Catalog # Description Quantity Price (USD)
Plasmid 63159 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Kabin Xie
  • Modifications to backbone
    Replace the 35S promoter with rice UBI promoter plus 5'-UTR.
  • Vector type
    Plant Expression, CRISPR
  • Promoter Rice snoRNA U3 promoter for gRNA/PTG expression and UBI promoter for Cas9 expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please use the reference links above to access a published protocol for using plasmids pRGE31, pRGEB31, pRGE32 and pRGEB32 as well as a CRISPR design software tool.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRGE32 was a gift from Yinong Yang (Addgene plasmid # 63159 ; ; RRID:Addgene_63159)
  • For your References section:

    Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system. Xie K, Minkenberg B, Yang Y. Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5. doi: 10.1073/pnas.1420294112. Epub 2015 Mar 2. 10.1073/pnas.1420294112 PubMed 25733849