Purpose(Empty Backbone) Expressing sgRNA and Cas9 in plants. The sgRNA was controlled by rice snoRNA U3 promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||50929||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size (bp) 6034
Modifications to backbone1. Removed Bsa I sites. 2. Insert a fragment containing Rice U3 promoter and gRNAscaffold 3. Insert Cas9 by LR reaction.
Vector typePlant Expression, CRISPR
- Promoter Rice snoRNA U3 and dual 35S promoter
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13R(-48)
- 3′ sequencing primer M13F(-20) (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe Cas9 fragment was from pX330.
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please use the reference links above to access a published protocol for using plasmids pRGE31, pRGEB31, pRGE32 and pRGEB32 as well as a CRISPR design software tool.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRGE31 was a gift from Yinong Yang (Addgene plasmid # 50929 ; http://n2t.net/addgene:50929 ; RRID:Addgene_50929)
For your References section:RNA-Guided Genome Editing in Plants Using a CRISPR-Cas System. Xie K, Yang Y. Mol Plant. 2013 Nov;6(6):1975-83. doi: 10.1093/mp/sst119. Epub 2013 Aug 17. 10.1093/mp/sst119 PubMed 23956122