Purpose(Empty Backbone) Binary vector derived from pRGE31. Deliver sgRNA and Cas9 into plant. by agrobacterium mediated transformation.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51295||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size (bp) 8958
Modifications to backbone1. Removed Bsa I sites. 2. Inserted the U3-gRNA-35S-Cas9 cassette from pRGE31.
Vector typePlant Expression, CRISPR ; plant expression
- Promoter Rice snoRNA U3 and dual 35S promoter
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Growth instructionsFor agrobacterium strains like EHA105, growth temperature is 28 C.
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13R(-48)
- 3′ sequencing primer M13F(-20) (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The full plasmid sequence was assembled, the XmaI site is not present.
Please use the reference links above to access a published protocol for using plasmids pRGE31, pRGEB31, pRGE32 and pRGEB32 as well as a CRISPR design software tool.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRGEB31 was a gift from Yinong Yang (Addgene plasmid # 51295 ; http://n2t.net/addgene:51295 ; RRID:Addgene_51295)
For your References section:RNA-Guided Genome Editing in Plants Using a CRISPR-Cas System. Xie K, Yang Y. Mol Plant. 2013 Nov;6(6):1975-83. doi: 10.1093/mp/sst119. Epub 2013 Aug 17. 10.1093/mp/sst119 PubMed 23956122