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pGTR
(Plasmid #63143)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 63143 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEM-T Easy
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3015
  • Total vector size (bp) 3192
  • Modifications to backbone
    Insert the gRNA-tRNA fused fragment by TA cloning
  • Vector type
    CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gRNA-tRNA fusion
  • Alt name
    GTR
  • Species
    Synthetic
  • Insert Size (bp)
    153

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGTR was a gift from Yinong Yang (Addgene plasmid # 63143 ; http://n2t.net/addgene:63143 ; RRID:Addgene_63143)
  • For your References section:

    Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system. Xie K, Minkenberg B, Yang Y. Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5. doi: 10.1073/pnas.1420294112. Epub 2015 Mar 2. 10.1073/pnas.1420294112 PubMed 25733849