164,102 results

1. pHCMV_SABV_GPC

   Plasmid

   #207305

   Purpose

   Mammalian expression of Sabia virus GPC protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   SABV_GPC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSETA_ionRD

   Plasmid

   #172924

   Purpose

   Ionic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.

   Depositor

   Arnold Boersma

   Article

   Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

   Insert

   IonicStrengthSensorRD
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT1QciMP

   Plasmid

   #62216

   Purpose

   QUAS transgenesis vector

   Depositor

   Harold Burgess

   Article

   Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

   Type

   Empty backbone

   Use

   Zebrafish transgenesis

   Tags

   Expression

   Mutation

   Promoter

   QUAS-E1b

   Available since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-GLT1b-V5

   Plasmid

   #162518

   Purpose

   Rat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loop

   Depositor

   Josef Kittler

   Article

   Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

   Insert

   GLT1b (Slc1a2 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-GLT1a-V5

   Plasmid

   #162517

   Purpose

   Rat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loop

   Depositor

   Josef Kittler

   Article

   Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

   Insert

   GLT1a (Slc1a2 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-ABE8.20m-nSpG-P2A-EGFP (KAC1160)

   Plasmid

   #185916

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8.20m-nSpG-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pv9_CBX1_2xChromo-13XL-BASU

   Plasmid

   #179412

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1.2xChromo-13XL-BASU

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX1.2xChromo-13XL-BASU
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. LCV2 STING KO

   Plasmid

   #217445

   Purpose

   Lentiviral vector expressing Cas9 and an sgRNA targeting human STING

   Depositor

   Jarrod Johnson

   Article

   Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

   Insert

   sgRNA targeting STING (STING1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti_Ef1a:PE2-P2A-GFP

   Plasmid

   #184445

   Purpose

   Single EF1a-driven PE2-P2A-GFP (Addgene #132776) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

   Depositor

   Bobby Koeleman

   Article

   Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Ef1A

   Available since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCF525-AcrIIA4_gb39_lenti

    Plasmid

    #138305

    Purpose

    Lenti expression of AcrIIA4

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    AcrIIA4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. p46Cpf1-OP2

    Plasmid

    #98592

    Purpose

    Produces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.

    Depositor

    Qiong Wu

    Article

    Ao et al Front Microbiol. 2018 Oct 9;9:2307. doi:

    Insert

    FnCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    araBAD

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pE-SUMO CRYGS

    Plasmid

    #80753

    Purpose

    Expression of human gamma S-crystallin in E. coli

    Depositor

    Larry David

    Insert

    CRYGS (CRYGS Human)
    

    Use

    Tags

    6xHIS and Smt3 SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP-CLC

    Plasmid

    #193559

    Purpose

    Expresses human Clathrin Light Chain B tagged with mEGFP in mammalian cells.

    Depositor

    Justin Taraska

    Article

    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

    Insert

    Clathrin light chain B (CLTB Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ssGNb-mCherry

    Plasmid

    #128788

    Purpose

    Mammalian expression

    Depositor

    Colin Taylor

    Article

    Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

    Insert

    ssGNb-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDH-puro-cMyc

    Plasmid

    #46970

    Purpose

    lentiviral expression of c-Myc

    Depositor

    Jialiang Wang

    Article

    Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

    Insert

    MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. NLS-tdPCP-GFP-VPR

    Plasmid

    #183936

    Purpose

    PCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFP

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    tdPCP-GFP-VPR
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV (TATA box removed)

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. C079

    Plasmid

    #174348

    Purpose

    Template for making mRNA for overexpressing eGFP in frog oocytes

    Depositor

    David Bartel

    Article

    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

    Insert

    eGFP
    

    Use

    In vitro transcription template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCherry-C1 SNX9 RYK mutant R286A Y287A K288A

    Plasmid

    #161993

    Purpose

    Mammalian expression vector containing a PIP-binding defective mutant of SNX9. Also contains mCherry tag

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

    Insert

    SNX9 (Snx9 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    R286A Y287A K288A

    Promoter

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. HDAC5 Flag

    Plasmid

    #13822

    Purpose

    Mammalian expression of human histone deacetylase 5 with flag tag

    Depositor

    Eric Verdin

    Article

    Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

    Insert

    HDAC5 (HDAC5 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Integrin β5-GFP

    Plasmid

    #205090

    Purpose

    Express GFP-tagged human integrin β5 in mammalian cells

    Depositor

    Bianxiao Cui

    Article

    Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

    Insert

    Integrin beta 5 (ITGB5 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge