182,008 results
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HA.PKD.S738E/S742E
Plasmid#10810DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPKD (PRKD1 Human)
TagsHAExpressionMammalianMutationActivation loop active: S738E and S742E (bp 2212-…PromoterCMVAvailable sinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-PE7-P2A-hMLH1dn
Plasmid#222997PurposeMammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPE7-P2A-hMLH1dn
TagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationDeletion of residues 754-756 in MLH1 as described…PromoterCMVAvailable sinceAug. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
COM-epegRNA-Dnmt1
Plasmid#211376PurposeDnmt1 targeting epegRNA with COM insertion in gRNA scaffold for v3b PE-eVLP productionDepositorInsertCOM-epegRNA
ExpressionMammalianAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS SARS-CoV-2 BA.4/5 Spike
Plasmid#186031PurposeExpresses codon-optimized full length SARS-CoV-2 BA.4/5 SpikeDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSARS-CoV-2 BA.4/5 Spike (S )
ExpressionMammalianMutationBA.2 mutations (T19I, LPPA24S, G142D, V213G, G339…Promoterchicken β-actin promoter, CMV enhancerAvailable sinceJune 8, 2022AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pET28 MBP_PrA/G-H6
Plasmid#176077PurposeExpress His6 tagged MBP_PrA/G in Escherichia coliDepositorInsertMBP PrA/G-H6
TagsHis tagExpressionBacterialPromoterT7 PromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D-TurboID(full)-HA-GPI
Plasmid#166055PurposeExpresses the full length TurboID on the surface of astrocytesDepositorInsertGPI anchored TurboID
UseAAVAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CaMKIIa-hM4D(Gi)-mCherry (AAV2)
Viral prep#50477-AAV2PurposeReady-to-use AAV2 particles produced from pAAV-CaMKIIa-hM4D(Gi)-mCherry (#50477). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hM4D(Gi)-mCherry plasmid DNA. CaMKIIa-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal silencing. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsmCherryAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOS007: PercevalHR ATP/ADP sensor (cytosolic)
Plasmid#163061PurposePercevalHR ATP/ADP sensor (cytosolic)DepositorInsertPercevalHR
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330.puro
Plasmid#110403PurposeCRISPR/Cas9 vector with sgRNA expression and puromycin resistanceDepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterCBhAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-N-Myc_WT USP7
Plasmid#131242PurposeMammalian expression of N-terminally Myc-tagged USP7DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
TagsMycExpressionMammalianPromoterCMV enhancer + CMV promoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SARS-CoV2 Nucleocapsid
Plasmid#153201PurposeExpresses myc-tagged nucleocapsid (N) from SARS-CoV2 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNucleocapsid from SARS-CoV-2 (N severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))
TagsMyc tag (5X)ExpressionMammalianPromoterCMVAvailable sinceJuly 15, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
SaKKH-ABE8e
Plasmid#138502PurposeA-to-G base editorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertecTadA(8e)-nSaCas9 (KKH)
ExpressionMammalianAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLJC5-3XMyc-EGFP-PEX26
Plasmid#139059PurposeMyc-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.DepositorInsert3XMyc-EGFP-PEX26
UseLentiviralTagsMycPromoterUBCAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EGFP-2xFYVE
Plasmid#136996PurposeLentiviral EGFP lipid sensor for PI(3)PDepositorInsertEGFP-2xFYVE
UseLentiviralTagsEGFPPromoterEF1 alphaAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-sp-sgRNA-RNF2_+41nick
Plasmid#135958PurposeS. pyogenes sgRNA for +41 nick in RNF2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRNF2_+41 nicking sgRNA
ExpressionMammalianMutationSee manuscriptPromoterU6Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyA
Plasmid#107307PurposeEncoding an SPH activation systemDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCAG-LSL-dCas9-10xGCN4-P2A-scFv-P65-HSF1-T2A-EGFP-WPRE-PolyA
ExpressionMammalianAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330 spCas9-mSA
Plasmid#113096PurposepX330 vector carrying spCas9-mSA for gene editing in mammalian cell linesDepositorInsertCAS9-MSA
UseSynthetic BiologyTagsMSA (monomeric streptavidinMutationnoneAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-U6-dgRNA-CAG-MPH
Plasmid#106259PurposeExpresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backboneDepositorAvailable sinceMay 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL3 3 kb prom. CD274
Plasmid#107004PurposeModified Luciferase expression vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCD274 (CD274 Human)
UseLuciferaseExpressionMammalianAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by
