DNA Polymerase iota Interacts with Both the TRAF-like and UBL1-2 Domains of USP7.
Ashton NW, Valles GJ, Jaiswal N, Bezsonova I, Woodgate R
J Mol Biol. 2021 Jan 22;433(2):166733. doi: 10.1016/j.jmb.2020.166733. Epub 2020 Dec 3. PubMed Article
Ashton NW, Valles GJ, Jaiswal N, Bezsonova I, Woodgate R
J Mol Biol. 2021 Jan 22;433(2):166733. doi: 10.1016/j.jmb.2020.166733. Epub 2020 Dec 3. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 131232 | pCMV6-AN-DDK_UBM1*2* POLI | Mammalian expression of an N-terminally FLAG-tagged DNA polymerase iota mutant, containing point mutations in both UBM domains |
| 131233 | pCMV6-AN-DDK_1-590 POLI | Mammalian expression of DNA polymerase iota residues 1-590 with an N-terminal FLAG-tag |
| 131234 | pCMV6-AN-DDK_1-420 POLI | Mammalian expression of DNA polymerase iota residues 1-420 with an N-terminal FLAG-tag |
| 131235 | pCMV6-AN-DDK_S433A POLI | Mammalian expression of S433A DNA polymerase iota with an N-terminal FLAG-tag |
| 131236 | pCMV6-AN-DDK_S497A POLI | Mammalian expression of S497A DNA polymerase iota with an N-terminal FLAG-tag |
| 131237 | pCMV6-AN-DDK_S520A POLI | Mammalian expression of S520A DNA polymerase iota with an N-terminal FLAG-tag |
| 131238 | pCMV6-AN-DDK_S580A POLI | Mammalian expression of S580A DNA polymerase iota with an N-terminal FLAG-tag |
| 131239 | pCMV6-AN-DDK_3KA POLI | Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A and K446A |
| 131240 | pCMV6-AN-DDK_RKK/AAA POLI | Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations R524A, K526A and K530A |
| 131241 | pCMV6-AN-DDK_3KA + S580A POLI | Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A, K446A and S580A |
| 131242 | pcDNA3.1-N-Myc_WT USP7 | Mammalian expression of N-terminally Myc-tagged USP7 |
| 131243 | pcDNA3.1-N-Myc_C223S USP7 | Mammalian expression of catalytically inactive Myc-tagged USP7 |
| 131244 | pcDNA3.1-N-Myc_1-560 USP7 | Mammalian expression of N-terminally Myc-tagged USP7, minus the UBL domains |
| 131245 | pcDNA3.1-N-Myc_1-205 USP7 | Mammalian expression of the USP7 TRAF-like domain with an N-terminal Myc tag. |
| 131246 | pcDNA3.1-N-Myc_535-1102 USP7 | Mammalian expression of the five USP7 UBL domains with an N-terminal Myc tag. |
| 131247 | pcDNA3.1-N-Myc_535-775 USP7 | Mammalian expression of the USP7 UBL1-2 domains with an N-terminal Myc tag. |
| 131248 | pcDNA3.1-N-Myc_535-888 USP7 | Mammalian expression of the USP7 UBL1-3 domains with an N-terminal Myc tag. |
| 131249 | pcDNA3.1-N-Myc_884-1102 USP7 | Mammalian expression of the USP7 UBL4-5 domains with an N-terminal Myc tag |
| 131250 | pcDNA3.1-N-Myc_775-1102 USP7 | Mammalian expression of the USP7 UBL3-5 domains with an N-terminal Myc tag |
| 131251 | pcDNA3.1-N-Myc_1-1083 USP7 | Mammalian expression of N-terminally Myc-tagged USP7, minus the C-terminal 18 amino acids |
| 131252 | pcDNA3.1-N-Myc_DW164AA USP7 | Mammalian expression of N-terminally Myc-tagged DW164AA USP7 |
| 131253 | pcDNA3.1-N-Myc_DED/AAA USP7 | Mammalian expression of an N-terminally Myc-tagged USP7 mutant, with mutations in UBL2 (DE758AA_D764A) |
| 131254 | pcDNA3.1-N-Myc_DW164AA 1-205 USP7 | Mammalian expression of a mutant USP7 TRAF-like domain (DW164AA), with an N-terminal Myc tag |
| 131255 | pcDNA3.1-N-Myc_DED/AAA 535-888 USP7 | Mammalian expression of USP7 UBL domains 1-3, with an N-terminal Myc tag and mutations in UBL2 (corresponding to DE758AA_D764A of the full-length protein) |
| 131256 | pcDNA3.1-N-Myc_DW164AA + DED/AAA USP7 | Mammalian expression of N-terminally Myc-tagged USP7, with mutations in the TRAF-like domain (DW164AA) and UBL2 (DE758AA_D764A) |
| 131257 | pX330_SpCas9_USP7 Exon 3 gRNA | pX330-U6-Chimeric_BB-Cbh-hSpCas9 vector expressing a guide RNA targeting exon 3 of USP7 |
| 131258 | pCMV6-AN-HA_Ubiquitin | Mammalian expression of ubiquitin with an N-terminal HA tag |