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pcDNA3.1-N-Myc_535-888 USP7
(Plasmid #131248)


Item Catalog # Description Quantity Price (USD)
Plasmid 131248 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5435
  • Total vector size (bp) 7154
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Ubiquitin carboxyl-terminal hydrolase 7
  • Alt name
    Ubiquitin-specific-processing protease 7
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    A mutant form of our pcDNA3.1-N-Myc_535-1102 USP7 plasmid (Addgene plasmid 131246), where the codon for E889 was converted to a STOP codon. This mutation limits expression to UBLs 1 - 3
  • Entrez Gene
    USP7 (a.k.a. C16DELp13.2, DEL16P13.2, HAFOUS, HAUSP, TEF1)
  • Tag / Fusion Protein
    • Myc (N terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-N-Myc_535-888 USP7 was a gift from Roger Woodgate (Addgene plasmid # 131248 ; ; RRID:Addgene_131248)
  • For your References section:

    DNA Polymerase iota Interacts with Both the TRAF-like and UBL1-2 Domains of USP7. Ashton NW, Valles GJ, Jaiswal N, Bezsonova I, Woodgate R. J Mol Biol. 2021 Jan 22;433(2):166733. doi: 10.1016/j.jmb.2020.166733. Epub 2020 Dec 3. 10.1016/j.jmb.2020.166733 PubMed 33279577