Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos.
Gu B, Posfai E, Rossant J
Nat Biotechnol. 2018 Jun 11. pii: nbt.4166. doi: 10.1038/nbt.4166.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
103882PCS2+Cas9-mSACas9 mSA for in vitro transcription for gene editing in Mouse Embryos
Loading...
112848EasyFusion eGFPGeneral donor vector with EGFP coding sequence flanking by restriction sites for generating knock-in donor
Loading...
112849EasyFusion mCherryGeneral donor vector with mCherry coding sequence flanking by restriction sites for generating knock-in donor
Loading...
112850EasyFusion HaloGeneral donor vector with HaloTag coding sequence flanking by restriction sites for generating knock-in donor
Loading...
112851EasyFusion T2A-H2B-GFPGeneral donor vector with T2A-H2B-GFP coding sequence flanking by restriction sites for generating knock-in donor
Loading...
112852EasyFusion Halo-mAIDGeneral donor vector with Halo-mAID coding sequence flanking by restriction sites for generating knock-in donor for Auxin inducible degradation of Halo tagged endogenous protein
Loading...
112853pR26-CAG-H2B-miRFP703Donor vector for targeting H2B-miRFP703 into Rosa26 locus to generate reporter mice or cell lines
Loading...
113086EasyFusion T2A-H2B-TagBFPGeneral donor vector with T2A-H2B-TagBFP coding sequence flanking by restriction sites for generating knock-in donor
Loading...
113089EasyFusion T2A-H2B-mCherryGeneral donor vector with T2A-H2B-mCherry coding sequence flanking by restriction sites for generating knock-in donor
Loading...
113096pX330 spCas9-mSApX330 vector carrying spCas9-mSA for gene editing in mammalian cell lines
Loading...
113097EasyFusion T2A-H2B-miRFP703General donor vector with T2A-H2B-miRFP703 coding sequence flanking by restriction sites for generating knock-in donor
Loading...
113103Ctcf-Halo-mAID donorDonor plasmid for knock-in mAID-Halo fusion to the c-terminal of mouse Ctcf coding sequence
Loading...
113104Halo-Erk2 donorDonor plasmid for knock-in HaloTag fusing to the N-terminal of mouse Erk2 coding sequence
Loading...
113113Cdk9-Halo donorDonor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Cdk9 coding sequence
Loading...
113115Gata4-GFP donorDonor plasmid for knock-in eGFP fusing to the c-terminal of mouse Gata4 coding sequence
Loading...
113116Id2-Halo donorDonor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Id2 coding sequence
Loading...
113117Fgfr2-T2A-H2B-Halo donorDonor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Fgfr2 coding sequence
Loading...
113118Dppa1-T2A-H2B-Halo donorDonor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Dppa1 coding sequence
Loading...
113119Gata3-T2A-H2B-eGFP donorDonor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Gata3 coding sequence
Loading...
113120Pdgfra-T2A-H2B-eGFP donorDonor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Pdgfra coding sequence
Loading...
113831EasyFusion mAID-mCherry with NeoEasyFusion vector for targeting endogeneous genes with mAID-mCherry
Loading...
113832PBS Actb-2A-TIR1-GFPtargeting osTIR1-GFP into mouse Actb locus for auxin inducible degradation
Loading...
122948PCS2+ Cas9For in vitro transcription to produce Cas9 mRNA for microinjection into mouse embryos
Loading...

Antibodies from Article